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Updated: Apr 18, 2026
Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining
Published on: June 23, 2020
Live-cell assessment of mitochondrial reactive oxygen species using dihydroethidine
Marleen Forkink1, Peter H G M Willems, Werner J H Koopman
1Department of Biochemistry, Raboud Institute for Molecular Life Sciences, Radboud University Medical Centre, P.O. Box 9101, NL-6500 HB, Nijmegen, The Netherlands.
Abstract:
Reactive oxygen species (ROS) play an important role in both physiology and pathology. Mitochondria are an important source of the primary ROS superoxide. However, accurate detection of mitochondrial superoxide especially in living cells remains a difficult task. Here, we describe a method and the pitfalls to detect superoxide in both mitochondria and the entire cell using dihydroethidium (HEt) and live-cell microscopy.
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