Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Potentiometry: Membrane Electrodes

Potentiometry: Membrane Electrodes

Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The peculiar properties of mitochondrial carriers of the SLC25 family.

The Biochemical journal·2025

Same author

Membrane potential stimulates ADP import and ATP export by the mitochondrial ADP/ATP carrier due to its positively charged binding site.

Science advances·2024

Same author

Human mitochondrial ADP/ATP carrier SLC25A4 operates with a ping-pong kinetic mechanism.

EMBO reports·2023

Same author

In Silico Modeling of the Mitochondrial Pumping Complexes with Markov State Models.

Methods in molecular biology (Clifton, N.J.)·2021

Same author

The proton pumping mechanism of the bc<sub>1</sub> complex.

Biochimica et biophysica acta. Bioenergetics·2020

Same author

Thermodynamic efficiency, reversibility, and degree of coupling in energy conservation by the mitochondrial respiratory chain.

Communications biology·2020

Search research articles

Related Experiment Video

Updated: Apr 18, 2026

Author Spotlight: New Insights into PBMC Mitochondrial Responses Using Fluorespirometry

Author Spotlight: New Insights into PBMC Mitochondrial Responses Using Fluorespirometry

Published on: May 24, 2024

Novel methods for measuring the mitochondrial membrane potential.

Roger Springett¹

¹Department of Radiology, Dartmouth Medical School, HB 7786, 1 Rope Ferry Road, Hanover, NH, 03755, USA, RSpringett@Dartmouth.edu.

Methods in Molecular Biology (Clifton, N.J.)

|January 30, 2015

Summary

Quantifying mitochondrial membrane potential is difficult. A new method uses the redox state of bc1 complex b-hemes, offering a more accurate way to measure this critical parameter for mitochondrial function.

More Related Videos

Multi-parameter Measurement of the Permeability Transition Pore Opening in Isolated Mouse Heart Mitochondria

Multi-parameter Measurement of the Permeability Transition Pore Opening in Isolated Mouse Heart Mitochondria

Published on: September 7, 2012

Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy

Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy

Published on: January 24, 2017

Related Experiment Videos

Last Updated: Apr 18, 2026

Author Spotlight: New Insights into PBMC Mitochondrial Responses Using Fluorespirometry

Author Spotlight: New Insights into PBMC Mitochondrial Responses Using Fluorespirometry

Published on: May 24, 2024

Multi-parameter Measurement of the Permeability Transition Pore Opening in Isolated Mouse Heart Mitochondria

Multi-parameter Measurement of the Permeability Transition Pore Opening in Isolated Mouse Heart Mitochondria

Published on: September 7, 2012

Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy

Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy

Published on: January 24, 2017

Area of Science:

Mitochondrial physiology and bioenergetics
Biophysical chemistry
Biochemical engineering

Background:

Mitochondrial membrane potential is vital for cellular energy production.
Current methods for measuring membrane potential rely on cation indicators, which have limitations.
Accurate quantification of mitochondrial membrane potential is essential for understanding cellular health and disease.

Purpose of the Study:

To introduce a novel methodology for quantifying mitochondrial membrane potential.
To establish the thermodynamic framework and algorithms for this new method.
To overcome the limitations of existing cation-dependent techniques.

Main Methods:

Utilizing the redox poise of b-hemes within the bc1 complex.
Measuring the oxidation states of specific b-hemes.
Applying thermodynamic principles and computational algorithms.

Main Results:

Developed a method to calculate mitochondrial membrane potential from b-heme redox states.
Established the thermodynamic basis for the new measurement technique.
Provided algorithms for data analysis.

Conclusions:

The novel method offers a promising alternative for accurate mitochondrial membrane potential measurement.
This approach bypasses the challenges associated with cation indicator accumulation.
Enables deeper insights into mitochondrial function and bioenergetics.