Related Experiment Video
Updated: Apr 18, 2026

Plant Growth and Agrobacterium-mediated Floral-dip Transformation of the Extremophyte Schrenkiella parvula
Published on: January 7, 2019
Chalcone synthase EaCHS1 from Eupatorium adenophorum functions in salt stress tolerance in tobacco
Chen Lijuan1, Guo Huiming, Lin Yi
1Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Key Message:
EaCHS1 functions in the tolerance of plantlets to salinity stress by maintaining ROS homeostasis. Chalcone synthase (CHS) is an essential enzyme in the biosynthesis of flavonoids. Expression of CHS is governed by a wide range of environmental stimuli, including UV light, pathogen attack, and circadian clocks. However, little research exists on the relationship between CHS and salinity stress. In this work, we constructed separate overexpression and RNA interference vectors of EaCHS1, and transferred them into tobacco. Overexpression of EaCHS1 increased the production of downstream flavonoids and the expressions of related genes in the phenylpropanoid pathway. It also improved resistance to salinity stress during seed germination and root development. In contrast, heterologous silencing of endogenous CHS in tobacco by a conserved EaCHS1 fragment had opposite effect. Together, our results indicated that changing the expression level of EaCHS1 in plants alters the accumulation of flavonoids and regulates plantlet tolerance to salinity stress by maintaining ROS homeostasis.
More Related Videos
08:27Analysis of Effect of Compound Salt Stress on Seed Germination and Salt Tolerance Analysis of Pepper Capsicum annuum L.
Published on: November 30, 2022
08:39Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress
Published on: October 11, 2024
Related Concept Videos
Responses to Salt Stress
Adaptations that Reduce Water Loss
Responses to Heat and Cold Stress
Regulation of Transpiration by Stomata
Cell Signaling in Plants
Responses to Drought and Flooding