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Related Experiment Video

Updated: Apr 18, 2026

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors
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iTRAQ-based quantitative proteomic analysis of cervical cancer.

Yibing Ding1, Min Yang2, Sha She2

  • 1Center for Translational Medicine, Nanjing University Medical School, Nanjing, P.R. China.

International Journal of Oncology
|January 31, 2015
PubMed
Summary

This study identified 294 distinct proteins in cervical cancer, revealing that reduced Glucose-6-phosphate dehydrogenase (G6PD) expression inhibits cancer cell migration and invasion, suggesting G6PD as a potential therapeutic target.

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Area of Science:

  • Oncology
  • Proteomics
  • Molecular Biology

Background:

  • Cervical cancer is a significant global health issue, ranking seventh overall and third among female cancers.
  • Current targeted therapies for cervical cancer require improvement through a deeper understanding of oncogenic protein profiles.

Purpose of the Study:

  • To systematically identify differentially expressed proteins in cervical cancer using advanced proteomic techniques.
  • To investigate the functional role of key identified proteins, particularly Glucose-6-phosphate dehydrogenase (G6PD), in cervical cancer progression.
  • To explore the potential of targeting specific proteins, like G6PD, for novel therapeutic strategies.

Main Methods:

  • iTRAQ labeling and NanoLC-MS/MS analysis were employed for comprehensive protein profiling.
  • Quantitative real-time PCR (qRT-PCR), western blot, and tissue microarray were used for validation.
  • RNA interference was utilized to functionally assess the role of G6PD in cervical cancer cells.

Main Results:

  • A total of 3,647 proteins were identified, with 294 proteins showing distinct expression levels in cervical cancer compared to non-tumor samples.
  • Key proteins including G6PD, ALDH3A1, STAT1, and HSPB1 were validated as differentially expressed.
  • Silencing G6PD expression in HeLa cells significantly reduced their in vitro migration and invasion capabilities.

Conclusions:

  • The study provides a comprehensive proteomic dataset that enhances the understanding of cervical cancer oncogenesis.
  • Glucose-6-phosphate dehydrogenase (G6PD) is identified as a highly expressed protein that promotes cervical cancer cell motility.
  • Inhibiting G6PD expression presents a promising therapeutic avenue for developing novel cervical cancer drugs.