Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Rapidly Varying Flow

Rapidly Varying Flow

Rapidly varying flow (RVF) in open channels is characterized by abrupt changes in flow depth over a short distance, with the rate of depth change relative to distance often approaching unity. These flows are inherently complex due to their transient and multi-dimensional nature, making exact analysis difficult. However, approximate solutions using simplified models provide valuable insights into their behavior.Key Features of Rapidly Varying FlowRVF is commonly observed in scenarios involving...

High-Performance Liquid Chromatography: Elution Process

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Development and evaluation of a model to predict hypersensitivity reaction when using 3HP for TB infection.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2026

Same author

Changes in the relationship between Index of Concentration at the Extremes and U.S. urban greenspace: a longitudinal analysis from 2001-2019.

Humanities & social sciences communications·2026

Same author

Non-Clostridium difficile Bacterial Infection in an Acute Flare of Crohn's Disease: A Case Report and Literature Review.

La Clinica terapeutica·2025

Same author

Distinctive Amelogenesis Imperfecta in Loeys-Dietz Syndrome Type II.

Journal of dental research·2025

Same author

Nuclear Astrophysics in the Storage Ring: Background Suppressed Simultaneous Measurement of (p,γ) and (p,n) Reactions.

Physical review letters·2025

Same author

Prognostic factor and risk stratification in hepatocellular carcinoma: insights from Cox regression and Kaplan-Meier analysis in a male-dominated cohort.

European review for medical and pharmacological sciences·2025

Same journal

Localization-driven exchange contrast in diffusion exchange spectroscopy.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026

Same journal

4.5 Tesla superconducting miniature magnet in liquid nitrogen.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026

Same journal

Folding and unfolding dynamics of a DNA aptamer studied by heteronuclear <sup>1</sup>H-<sup>13</sup>C correlation zz-exchange spectroscopy.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026

Same journal

Multi-spin control from one-spin pulses.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026

Same journal

Altering MRI rotating frame relaxations by changing the truncation level of Hyperbolic Secant pulse.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026

Same journal

Effects of proton exchange on the lifetimes of long-lived states in aliphatic chains.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 18, 2026

Rapid PCR Thermocycling using Microscale Thermal Convection

Rapid PCR Thermocycling using Microscale Thermal Convection

Published on: March 5, 2011

Gradient ascent pulse engineering for rapid exchange saturation transfer.

G Rancan¹, T T Nguyen², S J Glaser²

¹Department of Chemistry, Technical University Munich, 4, Lichtenbergstr., Garching, Germany; Klinikum rechts der Isar, Technical University Munich, 22, Ismaningerstr., München, Germany.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)

|January 31, 2015

Summary

This summary is machine-generated.

Optimizing saturation pulses for Yb-HPDO3A (a paraCEST agent) improves efficiency by 5-15% under energy constraints. This method enhances chemical exchange analysis for better clinical translation.

Keywords:

CEST GRAPE Nuclear magnetic resonance Optimal control ParaCEST Pulse engineering Yb-HPDO3A pH pH mapping

More Related Videos

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone

Published on: February 23, 2017

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling

Published on: October 28, 2014

Related Experiment Videos

Last Updated: Apr 18, 2026

Rapid PCR Thermocycling using Microscale Thermal Convection

Rapid PCR Thermocycling using Microscale Thermal Convection

Published on: March 5, 2011

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone

Published on: February 23, 2017

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling

Published on: October 28, 2014

Area of Science:

Magnetic Resonance Imaging
Biomedical Engineering
Chemical Exchange Saturation Transfer (CEST)

Background:

Clinical translation of Yb-HPDO3A (a paraCEST contrast agent) requires optimized saturation pulses.
Energy constraints are a critical factor in pulse design for clinical applications.

Purpose of the Study:

To investigate and optimize saturation pulse performance for Yb-HPDO3A under energy limitations.
To adapt the GRAPE algorithm for saturation pulse optimization considering chemical exchange.

Main Methods:

Adapted the GRAPE algorithm for saturation pulse optimization with chemical exchange.
Developed a methodology for extracting microscopic parameter ensembles.
Investigated rising amplitude waveforms for pulse design.
Validated pulse performance on a spectrometer and a clinical imager.

Main Results:

Optimal pulses demonstrated 5-15% higher saturation efficiencies compared to continuous wave methods.
Improved performance was observed especially for rapid and variable exchange rates (e.g., pH-catalysis).
No trade-offs in efficiency were identified.
Pool-specific optimized pulses enhanced CEST ratiometric analysis flexibility.

Conclusions:

The adapted GRAPE algorithm effectively optimizes saturation pulses for paraCEST agents like Yb-HPDO3A.
Optimized pulses offer significant efficiency gains under energy constraints, crucial for clinical translation.
The methodology allows for rapid testing and implementation of novel pulse designs.