Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Using caged neurotransmitters.

J J Marque1

  • 1Department of Engineering, Beckman Instruments, Palo Alto, California 94303-0803.

Nature
|February 9, 1989
PubMed
Summary

New instruments enable precise measurement of neuron responses to photolabile caged neurotransmitters. This advances the study of rapid receptor-ligand interactions in neuroscience.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Anesthetics and cerebral edema.

Anesthesiologyยท1976
See all related articles

Area of Science:

  • Neuroscience
  • Biochemistry
  • Biophysics

Background:

  • Photolabile caged neurotransmitters allow for precise temporal control over neurotransmitter release.
  • Studying the rapid kinetics of receptor-ligand interactions is crucial for understanding neural signaling.
  • Previous methods lacked the precision to capture split-second responses.

Purpose of the Study:

  • To develop an instrument for activating caged neurotransmitters.
  • To measure the resulting neuron response with high temporal resolution.
  • To facilitate the study of fast receptor-ligand binding kinetics.

Main Methods:

  • Development of a novel instrument integrating neurotransmitter activation and response measurement.
  • Utilized photolabile caged neurotransmitters for controlled release.
  • Recorded neuron electrical or biochemical responses following activation.

Main Results:

  • The instrument successfully activated caged neurotransmitters.
  • Precise measurement of neuron responses within split-second timescales was achieved.
  • Demonstrated the capability to study rapid receptor-ligand interactions.

Conclusions:

  • The developed instrument is a significant advancement for neuroscience research.
  • Enables detailed investigation of the kinetics of neurotransmitter receptor binding.
  • Opens new avenues for understanding neural processes at high speed.

Related Experiment Videos