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An analytical methodology can be divided into four sequential steps: technique, method, procedure, and protocol. A technique is a scientific principle that rationalizes a specific phenomenon through chemical measurements. Adapting a technique for analyzing a sample of interest is termed a method. The procedure outlines the directions for performing the analysis via an analytical method. The protocol is the detailed guidelines on the procedure, which should be strictly followed to obtain the...
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Method validation is a crucial process in analytical chemistry designed to confirm that a given method consistently produces reliable and high-quality results. This process is essential when a method is applied to different sample matrices or when procedural modifications are made, ensuring that the results meet acceptable standards across various applications.
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Standard solutions refer to solutions with a precisely known concentration or composition. A primary standard is a highly pure, high molar mass, stable substance that is entirely soluble in water, the most commonly used solvent in analytical chemistry. The primary standard solution can be used to standardize secondary standards, which are substances with known concentrations but are less pure and stable. Standard solutions are essential for achieving accurate and reliable results in analytical...
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In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
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Selection of reference standard during method development using the analytical hierarchy process.

Wan-yang Sun1, Ling Tong2, Dong-xiang Li2

  • 1School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.

Journal of Pharmaceutical and Biomedical Analysis
|January 31, 2015
PubMed
Summary
This summary is machine-generated.

Selecting the right reference standard is crucial for accurate analytical methods. This study introduces a quantitative approach using the analytical hierarchy process (AHP) to select the ideal reference standard for method development.

Keywords:
Analytical hierarchy processMethod developmentReference standardSalvia MiltiorrhizaUltra-performance liquid chromatography

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Area of Science:

  • Analytical Chemistry
  • Pharmacognosy
  • Decision Science

Background:

  • Reliable analytical methods depend on appropriate reference standards.
  • Selecting the optimal reference standard from available alternatives presents challenges due to immeasurable criteria.
  • Method development requires a systematic approach to reference standard selection.

Purpose of the Study:

  • To propose a quantitative method for selecting reference standards during analytical method development.
  • To utilize the analytical hierarchy process (AHP) as a decision-making tool for reference standard selection.
  • To evaluate the effectiveness of AHP in optimizing reference standard choices.

Main Methods:

  • Employed the analytical hierarchy process (AHP) for quantitative selection of reference standards.
  • Assessed six alternative single reference standards for analyzing phenolic acids in Salvia Miltiorrhiza.
  • Incorporated six criteria: feasibility, abundance, stability, accuracy, precision, and robustness.

Main Results:

  • Protocatechuic aldehyde was identified as the ideal reference standard.
  • Rosmarinic acid demonstrated 79.8% of the ideal standard's ability.
  • The AHP model effectively evaluated alternatives, considering benefits and risks.

Conclusions:

  • The analytical hierarchy process (AHP) provides an effective and practical tool for reference standard optimization.
  • This quantitative approach enhances the selection process for reference standards in method development.
  • The study validates AHP as a robust method for comprehensive decision-making in analytical chemistry.