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[Effect of high glucose on migration of BMSCs through inhibiting CXCR-4]
1Stomatological Center, General Hospital of PLA. Beijing 100853, China.E-mail:hugo_zhang_005@163.com.
Purpose:
To explore the effect of high glucose on migration of BMSCs through inhibiting CXCR-4.
Methods:
Bone marrow stromal cells (BMSCs) were obtained from the mandible of Wistar rats and stimulated with different concentrations of glucose (5.5, 16.5 mmol/L). The optimum concentration of SDF-1 was evaluated by Transwell assay in physiological glucose concentration (5.5 mmol/L). In the optimum concentration of SDF-1 condition, we detected the effect of SDF-1 and AMD3100 on migration of BMSCs in different concentrations of glucose (5.5, 16.5 mmol/L). CXCR-4 protein levels were determined by Western blot. The mRNA expression of CXCR-4 and MMP-2 were tested by RT-PCR. SPSS 11.0 software package was used for statistical analysis.
Results:
The optimum concentration of SDF-1 was 100 ng/mL. High glucose could inhibit the migration of BMSCs. In different concentrations of glucose, SDF-1 could promote the migration of BMSCs, but AMD3100 could inhibit this promotion. High glucose condition could inhibit the secretion of CXCR-4 and mRNA expression of CXCR-4 and MMP-2.
Conclusions:
High glucose inhibits migration of BMSCs by inhibiting CXCR-4 through SDF-1/CXCR-4 pathway.
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