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Cancer-Associated Fibroblasts from Mouse Mammary Tumors as Tools for Molecular and Computational Studies
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Histone demethylase JMJD2B and JMJD2C induce fibroblast growth factor 2: mediated tumorigenesis of osteosarcoma

Xiaojiang Li1, Shanshan Dong

  • 1Orthopedics Department, The Affiliated Hospital to Changchun University of Chinese Medicine, No.1478 of Gongnong Street, Chaoyang District, Changchun, 130021, China, xiaojianglicc@163.com.

Medical Oncology (Northwood, London, England)
|February 1, 2015
PubMed

Insights

Histone demethylases JMJD2B and JMJD2C are elevated in osteosarcoma, promoting cancer progression by up-regulating Fibroblast Growth Factor 2 (FGF2). Targeting JMJD2B and JMJD2C may offer new osteosarcoma therapies.

Area of Science:

  • Oncology
  • Molecular Biology
  • Epigenetics

Background:

  • Histone demethylases JMJD2B and JMJD2C are implicated in various cancers.
  • Their specific roles in osteosarcoma pathogenesis are not well understood.
  • Osteosarcoma aggressiveness is linked to factors like Fibroblast Growth Factor 2 (FGF2).

Purpose of the Study:

  • To investigate the expression and function of JMJD2B and JMJD2C in osteosarcoma.
  • To determine the relationship between JMJD2B/JMJD2C and osteosarcoma cell behavior.
  • To elucidate the molecular mechanism involving JMJD2B/JMJD2C and FGF2 in osteosarcoma.

Main Methods:

  • Quantitative real-time PCR and Western Blotting to assess JMJD2B/JMJD2C levels.
  • Analysis of JMJD2B/JMJD2C expression in osteosarcoma tissues versus adjacent non-tumor tissues.
  • GST pull-down assays to confirm protein interactions.

Main Results:

  • JMJD2B and JMJD2C expression was significantly higher in osteosarcoma tissues compared to normal tissues.
  • Elevated JMJD2B/JMJD2C levels correlated with increased osteosarcoma cell metastasis.
  • JMJD2B/JMJD2C directly up-regulated FGF2, which in turn promoted osteosarcoma cell proliferation, migration, and invasion.

Conclusions:

  • JMJD2B and JMJD2C play a critical role in osteosarcoma progression through the FGF2 pathway.
  • These histone demethylases are potential therapeutic targets for osteosarcoma treatment.

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