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Updated: Apr 18, 2026

An In Ovo Model for Testing Insulin-mimetic Compounds
Published on: April 23, 2018
In vitro anti-hyperglycemic activity of 4-hydroxyisoleucine derivatives
Venkateswarlu Korthikunta1, Jyotsana Pandey2, Rohit Singh1
1Medicinal and Process Chemistry Division, CSIR-Central Drug Research Institute, Lucknow, UP 226 031, India.
Abstract:
The nonproteinogenic amino acid, 4-hydroxyisoleucine (1) has been isolated in large quantities from the fenugreek (T. foenum-graecum) seeds. Few novel derivatives (3-11 and 13-18) were prepared from the naturally occurring 4-hydroxyisoleucine (1) and screened for their in vitro glucose uptake stimulatory effect in L-6 skeletal muscle cells. The derivatives 6, 7, 8, 10 and 11 exhibited better glucose uptake stimulatory activity than parent compound, 4-hydroxyisoleucine at 5 and 10µM concentrations and compounds 7 and 11 enhanced translocation of insulin sensitive glucose transporters-4 in skeletal muscle cells.
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