HEpD: a database describing epigenetic differences between Thoroughbred and Jeju horses

Area of Science:

• Equine genomics
• Epigenetics
• Comparative biology

Background:

• Next-generation sequencing enables genome-wide DNA methylation mapping.
• Thoroughbred and Jeju horses possess distinct breeding histories and traits.

Purpose of the Study:

• To develop a database of genome-wide DNA methylation patterns in Thoroughbred and Jeju horses.
• To investigate epigenetic differences underlying athletic traits in equine breeds.

Main Methods:

• Utilized Methylated DNA Immunoprecipitation sequencing (MeDIP-Seq).
• Analyzed DNA methylation in cerebrum, lung, heart, and skeletal muscle tissues.
• Identified significantly enriched methylated regions and differentially methylated regions (DMRs).

Main Results:

• Generated genome-wide DNA methylation profiles for key equine organs.
• Provided data on methylation density and DMRs across 784 gene regions.
• Highlighted potential epigenetic variations between Thoroughbred and Jeju horses.

Conclusions:

• The developed database offers valuable insights into equine epigenetics.
• Facilitates research into breed-specific differences and genetic underpinnings of athletic performance.
• Supports further investigation of DMRs in equine tissues.