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Updated: Apr 18, 2026

Preparation of Cell-lines for Conditional Knockdown of Gene Expression and Measurement of the Knockdown Effects on E4orf4-Induced Cell Death
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Preparation of Cell-lines for Conditional Knockdown of Gene Expression and Measurement of the Knockdown Effects on E4orf4-Induced Cell Death

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Adenovirus encoding Smad4 suppresses glioma cell proliferation and increases apoptosis through cell cycle arrest at

Zhao Yang1, Lina Zhong1, Shanchuan Zhong1

  • 1Department of Neurology, Yongchuan Hospital, Chongqing Medical, University, Chongqing 402160, China.

International Immunopharmacology
|February 3, 2015
PubMed

Insights

Mothers against decapentaplegic homologue 4 (Smad4) suppresses glioma cell proliferation and increases apoptosis. Overexpressing Smad4 in glioma may offer a potential therapeutic strategy for this brain cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Mothers against decapentaplegic homologue 4 (Smad4) is implicated in various human cancers.
  • The precise role of Smad4 in human glioma pathogenesis remains unclear.

Purpose of the Study:

  • To investigate the functional role of Smad4 in human glioma.
  • To explore Smad4 as a potential therapeutic target for glioma.

Main Methods:

  • Constructed a recombinant adenovirus encoding Smad4.
  • Transduced Smad4 into human glioma cells.
  • Evaluated effects on cell proliferation, apoptosis, and cell cycle progression.
  • Assessed tumor formation in a nude mouse xenograft model.

Main Results:

  • Overexpression of Smad4 significantly suppressed glioma cell proliferation.
  • Smad4 overexpression induced cell apoptosis by promoting G1 phase cell cycle arrest.
  • Adenovirus-mediated Smad4 delivery inhibited tumor formation in vivo.

Conclusions:

  • Smad4 plays a critical role in regulating glioma cell proliferation and survival.
  • Smad4 demonstrates potential as a therapeutic target for human glioma treatment.
Keywords:
AdenovirusGlioma cellProliferationSmad4

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