Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Patch Clamp01:18

Patch Clamp

7.7K
Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...
7.7K
Electrophysiology of Normal Cardiac Rhythm01:19

Electrophysiology of Normal Cardiac Rhythm

10.6K
The normal cardiac rhythm is a synchronized electrical activity that facilitates the regular and coordinated contraction of the heart muscle. This process is essential for efficient blood circulation throughout the body. The fundamental elements involved in establishing and maintaining this rhythm include the unique electrical properties of cardiac muscle cells, the sinoatrial (SA) node's pacemaker function, the specialized conducting system, and the ionic mechanisms underlying each phase...
10.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Long-Read Nanopore Sequencing Enhances BRCA1/2 Variant Detection Compared with Ion Torrent Analysis.

The Journal of molecular diagnostics : JMD·2026
Same author

Quantifying intracellular mechanosensitive response upon spatially defined mechano-chemical triggering.

eLife·2026
Same author

Temperature-dependent ligand relocation reveals plasticity of TRPM4 inhibition.

bioRxiv : the preprint server for biology·2026
Same author

Biochemical assessment of α-α-subunit interactions of Na<sub>v</sub>1.5 in a heterologous expression system.

Scientific reports·2026
Same author

Glycosylation of the murine cardiac channel TRPM4 is altered by the pathogenic p.I376T variant.

Experimental physiology·2026
Same author

An integrated <i>i</i> <i>n vitro</i> platform and biophysical modeling approach for studying synaptic transmission in isolated neuronal pairs.

iScience·2026

Related Experiment Video

Updated: Apr 18, 2026

Electromechanical Assessment of Optogenetically Modulated Cardiomyocyte Activity
12:52

Electromechanical Assessment of Optogenetically Modulated Cardiomyocyte Activity

Published on: March 5, 2020

9.0K

Force-controlled patch clamp of beating cardiac cells.

Dario Ossola1, Mohamed-Yassine Amarouch, Pascal Behr

  • 1Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich , Zurich, Switzerland.

Nano Letters
|February 3, 2015
PubMed
Summary
This summary is machine-generated.

This study integrates atomic force microscopy (AFM) with patch clamp electrophysiology, enabling simultaneous force and electrical recordings. This novel approach enhances ion channel research and cell function analysis with improved precision and gentleness.

Keywords:
FluidFMNaV1.5 channelsWhole-cell patch clampatomic force microscopecardiomyocytesmicrochanneled AFM cantilevers

More Related Videos

Isolation, Culture, and Functional Characterization of Adult Mouse Cardiomyoctyes
12:49

Isolation, Culture, and Functional Characterization of Adult Mouse Cardiomyoctyes

Published on: September 24, 2013

25.6K
Voltage-Dependent Potassium Current Recording on H9c2 Cardiomyocytes via the Whole-Cell Patch-Clamp Technique
08:11

Voltage-Dependent Potassium Current Recording on H9c2 Cardiomyocytes via the Whole-Cell Patch-Clamp Technique

Published on: November 11, 2022

3.7K

Related Experiment Videos

Last Updated: Apr 18, 2026

Electromechanical Assessment of Optogenetically Modulated Cardiomyocyte Activity
12:52

Electromechanical Assessment of Optogenetically Modulated Cardiomyocyte Activity

Published on: March 5, 2020

9.0K
Isolation, Culture, and Functional Characterization of Adult Mouse Cardiomyoctyes
12:49

Isolation, Culture, and Functional Characterization of Adult Mouse Cardiomyoctyes

Published on: September 24, 2013

25.6K
Voltage-Dependent Potassium Current Recording on H9c2 Cardiomyocytes via the Whole-Cell Patch-Clamp Technique
08:11

Voltage-Dependent Potassium Current Recording on H9c2 Cardiomyocytes via the Whole-Cell Patch-Clamp Technique

Published on: November 11, 2022

3.7K

Area of Science:

  • Biophysics
  • Electrophysiology
  • Cell Biology

Background:

  • Patch clamp is the gold standard for electrophysiology research and drug screening.
  • Voltage-gated ion channels are crucial for action potentials and cellular function.
  • Existing automated patch clamp systems lack the versatility of conventional methods for fundamental research.

Purpose of the Study:

  • To merge patch clamp technique with atomic force microscopy (AFM) for enhanced electrophysiological measurements.
  • To equip patch clamp with sensitive AFM force control for precise cell interaction.
  • To demonstrate the feasibility of simultaneous electrical and force recordings in cellular systems.

Main Methods:

  • Utilized FluidFM, a force-controlled nanopipette based on microchanneled AFM cantilevers.
  • Integrated FluidFM with standard patch-clamp electronics.
  • Performed whole-cell recordings of voltage-gated sodium channels (NaV1.5).
  • Simultaneously recorded membrane current and force development in isolated cardiomyocytes.

Main Results:

  • Demonstrated compatibility of the merged system with patch-clamp electronics.
  • Successfully recorded activity of voltage-gated ion channels (NaV1.5) in whole-cell configuration.
  • Achieved simultaneous recording of membrane current and force development during cardiomyocyte contraction.
  • Showcased gentle and stable cell-membrane contact using force feedback for serial patch clamping and injection without cell damage.

Conclusions:

  • The integration of AFM force control with patch clamp electrophysiology offers a powerful new tool for fundamental research.
  • This hybrid technique enables precise, simultaneous measurement of electrical activity and mechanical forces at the cellular level.
  • The system facilitates advanced applications like serial patch clamping and intracellular injection with minimal cell perturbation.