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Updated: Apr 18, 2026
In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
Published on: August 23, 2019
MiR-429 inhibits oral squamous cell carcinoma growth by targeting ZEB1
Wanke Lei1, Yun-e Liu2, Yuzhu Zheng3
1Department of Stomatology, People's Hospital of Mianzhu, Mianzhu, Sichuan, China (mainland).
Background:
Oral squamous cell carcinoma (OSCC) is the sixth most common human malignancy worldwide. To develop new therapeutics requires elucidation of the underlying mechanism of OSCC pathogenesis. The role of miR-429 in OSCC remains unknown.
Material/Methods:
The level of miR-429 and ZEB1 in OSCC tissues and cell lines was measured by qRT-PCR. MiR-429 was down-regulated by miRNAs antisense oligonucleotides (ASO) transfection and up-regulated by miRNAs mimics. Cell proliferation was analyzed by MTT assay. Cell apoptosis was revealed by FACS analysis. Targeted genes were predicted by a bioinformatics algorithm and confirmed by a dual luciferase reporter assay.
Results:
MiR-429 was down-regulated in OSCC tissues, and miR-429 overexpression inhibited OSCC cell lines growth and vice versa. Further, we found that miR-429 could inhibit zinc finger E-boxbinding homeobox 1 (ZEB1) expression, and that miR-429 and ZEB1 expression in OSCC tissues were negatively correlated.
Conclusions:
Our data demonstrate the tumor suppressor role of miR-429 in OSCC, and may provide a potential therapeutic target that warrants further investigation.
Insights
MicroRNA-429 (miR-429) acts as a tumor suppressor in oral squamous cell carcinoma (OSCC) by inhibiting ZEB1 expression. This finding offers a potential new therapeutic target for OSCC treatment.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Oral squamous cell carcinoma (OSCC) is a prevalent global malignancy.
- Understanding OSCC pathogenesis is crucial for developing novel therapeutics.
- The specific role of miR-429 in OSCC was previously uncharacterized.
Purpose of the Study:
- To investigate the role of miR-429 in the pathogenesis of oral squamous cell carcinoma.
- To determine the relationship between miR-429 and ZEB1 in OSCC.
- To evaluate miR-429 as a potential therapeutic target for OSCC.
Main Methods:
- Quantitative real-time PCR (qRT-PCR) to measure miR-429 and ZEB1 levels.
- Manipulation of miR-429 expression using antisense oligonucleotides (ASO) and miRNA mimics.
- Assessment of cell proliferation via MTT assay and apoptosis via FACS analysis.
- Bioinformatic prediction and dual-luciferase reporter assay to confirm ZEB1 as a target gene.
Main Results:
- MiR-429 expression was significantly down-regulated in OSCC tissues.
- Overexpression of miR-429 inhibited OSCC cell growth and induced apoptosis.
- MiR-429 directly targets and inhibits the expression of zinc finger E-box-binding homeobox 1 (ZEB1).
- A negative correlation was observed between miR-429 and ZEB1 expression in OSCC tissues.
Conclusions:
- MiR-429 exhibits a tumor suppressor function in oral squamous cell carcinoma.
- The miR-429/ZEB1 axis represents a potential therapeutic strategy for OSCC.
- Further investigation into miR-429 as a therapeutic target for OSCC is warranted.
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