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Updated: Apr 18, 2026
Murine Model of Wound Healing
Published on: May 28, 2013
Evaluation of a topical herbal patch for soft tissue wound healing: an animal study
Liat Chaushu1, Miron Weinreb, Ilan Beitlitum
1Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
Aim:
This study evaluated the effects of a topical herbal patch (PerioPatch®) for gingival wound healing in a rat model.
Materials And Methods:
A mid-crestal incision was performed on each side of the edentulous anterior maxilla in 48, 6-month-old, Wistar rats. Full-thickness flaps were raised, repositioned and sutured. Four experimental groups were established: herbal patch, placebo patch, no patch and no patch and no surgery. Patches were placed immediately after surgery and replaced every 12 h for the following 3 days. Half of the animals were killed after 5 and the remaining ones after 12 days. Tissue blocks were retrieved and processed for histological and immunohistochemical evaluation. Epithelial gap, collagen contents, amount of macrophages, cellular proliferation and vascular contents were evaluated in the central incision area. Statistical analysis consisted of two-way anova.
Results:
The herbal patch group presented the smallest epithelial gap at 12 days, the highest collagen content both at 5 and 12 days, a larger number of proliferating cells at day 5 and more numerous blood vessels at day 12. Macrophage number was similar in all groups.
Conclusion:
Herbal patch improved wound healing in this animal model.
