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Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing
Published on: October 3, 2018
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Ligand-directed profiling of organelles with internalizing phage libraries
Andrey S Dobroff1,2,3, Roberto Rangel4,3, Liliana Guzman-Roja4,3
1Division of Hematology/Oncology, Department of Internal Medicine, University of New Mexico School of Medicine, Albuquerque, New Mexico.
Current Protocols in Protein Science
|February 3, 2015
Summary
Internalizing phage (iPhage) technology enables unbiased discovery of protein interactions and development of targeted therapies. This method identifies internalizing homing peptides for cell biology and drug development applications.
Area of Science:
- Biotechnology
- Molecular Biology
- Cell Biology
Background:
- Phage display is a versatile technology for protein engineering and interaction discovery.
- Existing methods lack efficient tools for intracellular targeting and pathway analysis.
Purpose of the Study:
- To introduce and describe a novel internalizing phage (iPhage) display system.
- To enable ligand-directed targeting of organelles and identification of molecular pathways within live cells.
- To provide methods for generating, screening, and validating iPhage for therapeutic and diagnostic applications.
Main Methods:
- Development of a new class of internalizing phage (iPhage).
- Screening of iPhage libraries for specific cellular uptake and targeting.
- Selection and validation of internalizing homing peptides.
Main Results:
- The iPhage system allows for unbiased discovery of functional protein-protein interactions.
- iPhage facilitates targeted delivery to specific organelles within live cells.
- Validated internalizing homing peptides demonstrate potential for drug development.
Conclusions:
- The iPhage display system offers a powerful platform for fundamental cell biology research.
- This technology has significant implications for developing targeted diagnostic and therapeutic agents.
- iPhage technology bridges basic research and drug development applications.
