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A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination
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FADD phosphorylation impaired islet morphology and function.

Chun Yao1, Hongqin Zhuang, Wei Cheng

  • 1The State Key Laboratory of Pharmaceutical Biotechnology, School of Stomatology and Affiliated Stomatological Hospital, Nanjing University, Nanjing, 210093, P. R. China.

Journal of Cellular Physiology
|February 3, 2015
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Fas-associated death domain-containing protein (FADD) phosphorylation impacts islet development and insulin secretion. Constitutively phosphorylated FADD in mice led to smaller islets and impaired glucose-stimulated insulin secretion.

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Area of Science:

  • Cell biology
  • Endocrinology
  • Molecular signaling

Background:

  • The Fas-FasL pathway and caspase-8 regulate islet mass and insulin secretion.
  • Fas-associated death domain-containing protein (FADD), a key adaptor in Fas-FasL signaling, participates in non-apoptotic processes modulated by its phosphorylation.
  • The specific role of FADD in pancreatic islets remains unexplored.

Purpose of the Study:

  • To investigate the role of FADD phosphorylation in pancreatic islet development and function.
  • To identify proteins regulated by FADD phosphorylation that are involved in islet differentiation and function.

Main Methods:

  • Comparative proteomics and bioinformatic analysis of phosphorylated FADD (FADD-D) and wild-type (WT) MEFs.
  • Generation and analysis of a mouse model with constitutive phosphorylated FADD (FADD-D).
  • Immunohistological analysis of pancreatic islets from FADD-D mice.

Main Results:

  • FADD phosphorylation dysregulated three proteins crucial for islet differentiation and function.
  • FADD-D mice exhibited reduced islet area compared to WT controls.
  • Impaired glucose-stimulated insulin secretion (GSIS) was observed in islets from FADD-D mice.

Conclusions:

  • FADD phosphorylation plays a significant role in regulating pancreatic islet development.
  • FADD is implicated in controlling insulin secretion.
  • These findings reveal a novel function for FADD in islet biology.