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Rapid analysis of forensic samples using an atmospheric solid analysis probe interfaced to a linear ion trap mass
Eshwar Jagerdeo1, Jay A Clark, Jeffrey N Leibowitz
1Federal Bureau of Investigation Laboratory, Quantico, VA, 22135, USA.
Rapid Communications in Mass Spectrometry : RCM
|February 3, 2015
Summary
Atmospheric Solid Analysis Probe (ASAP) coupled with a mass spectrometer enables rapid analysis of diverse samples with minimal preparation. This technique provides high-quality data for routine analysis and confirmation using MS/MS libraries.
Area of Science:
- Analytical Chemistry
- Mass Spectrometry
Background:
- Highlights the simplicity of interfacing an Atmospheric Solid Analysis Probe (ASAP) to a Linear Ion Trap Mass Spectrometer.
- Demonstrates rapid generation of high-quality data from diverse sample types with minimal or no sample preparation.
Purpose of the Study:
- To showcase the utility of ASAP coupled with a Linear Ion Trap Mass Spectrometer for rapid sample analysis.
- To validate the technique for direct analysis of various solid and liquid samples.
Main Methods:
- Solid samples are analyzed by rubbing a capillary melting tube on the sample surface and introducing it into the mass spectrometer source.
- Liquid samples are analyzed by dipping a capillary tube into the sample or applying microliters to the capillary tip.
- Analysis is performed using Atmospheric Pressure Chemical Ionization in both positive and negative modes, with detection by full scan mass spectrometry (MS) and confirmation by MS/MS.
Main Results:
- Successfully analyzed a rodenticide (brodifacoum), heroin and its impurities, crack cocaine, and a dyestuff without sample preparation.
- All compounds were detected using full scan MS, with confirmation via MS/MS.
- Preliminary results indicate potential for quantitative analysis.
Conclusions:
- Interfacing ASAP with an ion trap mass spectrometer enables full scan, MS(n) experiments, and rapid positive/negative switching.
- The instrument is highly effective for rapid, routine analysis and confirmation using in-house MS/MS libraries.
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