Related Experiment Video
Updated: Apr 18, 2026

A Time-Efficient Fluorescence Spectroscopy-Based Assay for Evaluating Actin Polymerization Status in Rodent and Human Brain Tissues
Published on: June 3, 2021
Relationship of light scatter change and Cdc42-regulated actin status
Lin Hong1,2, Stephanie Chavez1, Yelena Smagley1
1Department of Pathology, The University of New Mexico, Albuquerque, New Mexico, 87131.
Background:
Cdc42 GTPase has important roles in regulating intracellular actin reorganization. The current methods to monitor actin changes are typically complex and point by point.
Methods:
The effects of Cdc42 inhibitors on the side scatter changes were tested in a newly developed continuous assay using the flow cytometer. Staining with fluorescently labeled phalloidin was used for comparison.
Results:
Cdc42-specific inhibitors caused dose-dependent changes of both the right-angle side scatter and the phalloidin-stained actin.
Conclusions:
The right-angle light scatter change can be used as a method to circumvent phalloidin staining and be an early convenient step in screening Cdc42 inhibitors. © 2015 International Clinical Cytometry Society.
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