Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

102.2K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
102.2K
RNA-seq03:21

RNA-seq

12.7K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
12.7K
DNA Isolation01:24

DNA Isolation

47.2K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
47.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Programmable one-pot polymerase-mediated DNA synthesis via temperature control.

Nature communications·2026
Same author

Gut microbiota transfer from autoimmune dry eye mice imprints stereotypic B cell receptor repertoires in the lacrimal gland and induces disease.

Frontiers in immunology·2026
Same author

Spatial multi-omics reveals region-specific molecular signatures in a 6-OHDA model of Parkinson's disease.

NPJ Parkinson's disease·2026
Same author

Spatial distribution of rare earth elements in seawater and sediments, and bioaccumulation in plankton from the Yellow Sea.

Marine pollution bulletin·2026
Same author

A large-scale vision foundation model for musculoskeletal radiographs.

NPJ digital medicine·2026
Same author

Rare Anti-f(ce) Detected During Cross-Matching.

Clinical laboratory·2026

Related Experiment Video

Updated: Apr 18, 2026

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

6.3K

A high-throughput optomechanical retrieval method for sequence-verified clonal DNA from the NGS platform.

Howon Lee1, Hyoki Kim2, Sungsik Kim3

  • 1Institutes of Entrepreneurial BioConvergence, Seoul National University, Seoul 151-742, Republic of Korea.

Nature Communications
|February 3, 2015
PubMed
Summary

Sniper Cloning enables precise mapping and retrieval of DNA clones from next-generation sequencing (NGS) platforms. This DNA writing technology efficiently produces thousands of error-free synthetic DNA molecules in a single run.

More Related Videos

gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair
08:15

gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair

Published on: October 6, 2014

12.8K
Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens
08:49

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Published on: June 6, 2020

15.6K

Related Experiment Videos

Last Updated: Apr 18, 2026

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

6.3K
gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair
08:15

gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair

Published on: October 6, 2014

12.8K
Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens
08:49

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Published on: June 6, 2020

15.6K

Area of Science:

  • Synthetic Biology
  • Bioengineering
  • Functional Genomics

Background:

  • DNA writing is crucial for synthetic biology, functional genomics, and bioengineering.
  • Next-generation sequencing (NGS) platforms offer a potential source of sequence-verified DNA for DNA writing.
  • Retrieving specific DNA clones from high-density NGS platforms remains a significant challenge.

Purpose of the Study:

  • To develop an enabling technology for precise mapping and non-contact retrieval of target DNA clones from NGS platforms.
  • To facilitate the full utilization of DNA clones for DNA writing applications.
  • To establish a cost-effective and efficient method for producing synthetic DNA.

Main Methods:

  • Integration of next-generation sequencing (NGS), DNA microarray, and a pulse laser retrieval system.
  • Development of 'Sniper Cloning' for precise mapping of target clone features on NGS platforms.
  • Non-contact rapid retrieval of target DNA clones.

Main Results:

  • Sniper Cloning enables precise mapping and retrieval of target DNA clones.
  • The technology successfully produced 5,188 error-free synthetic DNAs in a single, week-long run.
  • Achieved efficient utilization of DNA clones from a single microarray DNA pool on an NGS platform.

Conclusions:

  • Sniper Cloning is a novel and efficient technology for DNA writing.
  • This method overcomes the challenge of retrieving target clonal DNA from high-density NGS platforms.
  • Sniper Cloning has the potential to become a universal tool in biological sciences for DNA synthesis and engineering.