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Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

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Updated: Apr 18, 2026

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

Challenges in DNA motion control and sequence readout using nanopore devices.

Spencer Carson¹, Meni Wanunu

¹Department of Physics, Northeastern University, Boston, MA 02115, USA.

|February 3, 2015

Summary

This summary is machine-generated.

Nanopore sequencing offers cheap, high-throughput DNA analysis. This review details sensing techniques and methods, like biological motors, to control DNA translocation for reliable sequencing.

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Last Updated: Apr 18, 2026

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Published on: December 14, 2017

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

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Published on: October 31, 2013

Area of Science:

Biotechnology
Genomics
Nanotechnology

Background:

Nanopore technology is a promising platform for next-generation DNA sequencing.
Current nanopore sequencing faces challenges with DNA translocation speed and stochasticity.

Purpose of the Study:

To review sensing techniques for DNA sequencing and mapping using nanopores.
To discuss strategies for controlling DNA translocation in nanopore analysis.

Main Methods:

Review of existing literature on nanopore sensing techniques.
Analysis of methods to control DNA translocation, including biological motors and experimental modifications.

Main Results:

Various sensing techniques are under investigation for nanopore DNA analysis.
Biological motors and pore modifications are explored to manage DNA translocation.

Conclusions:

Controlling DNA translocation is critical for reliable nanopore sequencing.
Further research into sensing techniques and translocation control is needed for nanopore applications.