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Related Concept Videos

Proteomics01:33

Proteomics

10.2K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Related Experiment Video

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Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry
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Proteomics-driven analysis of ovine whey colostrum.

Domenica Scumaci1, Francesca Trimboli2, Ludovica Dell'Aquila3

  • 1Dpt. of Experimental and Clinical Medicine, Magna Græcia University of Catanzaro, Catanzaro, Italy.

Plos One
|February 3, 2015
PubMed
Summary
This summary is machine-generated.

This study comprehensively analyzed ovine colostrum proteins, identifying 343 unique proteins. The research highlights the importance of low-abundance proteins for newborn lamb development.

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Area of Science:

  • Animal Science
  • Proteomics
  • Biochemistry

Background:

  • Ovine colostrum contains dominant proteins like immunoglobulins and less abundant, crucial proteins for neonates.
  • Understanding the complete ovine colostrum proteome is vital for neonatal development.

Purpose of the Study:

  • To comprehensively analyze the ovine colostrum proteome.
  • To identify low-abundance proteins and their potential roles.

Main Methods:

  • Ovine colostrum was collected post-lambing.
  • Proteins were separated using 1D SDS-PAGE.
  • Peptide analysis was performed using LC-MS/MS and Swiss-Prot database searching.

Main Results:

  • Identification of 343 unique proteins in ovine colostrum.
  • Detailed characterization of the ovine colostrum protein composition.
  • This represents the most extensive proteomic analysis of ovine colostrum to date.

Conclusions:

  • The ovine colostrum proteome is complex, with numerous low-abundance proteins.
  • These proteins play significant roles in neonatal development.
  • This comprehensive proteomic dataset provides a foundation for future research.