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A Fluorescence-based Protocol for Preliminary Screening of Protein Synthesis Inhibitors from Natural Sources

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Imine (-CH=N-) brings selectivity for silver enhanced fluorescence.

Mainak Ganguly¹, Jaya Pal, Chanchal Mondal

¹Department of Chemistry, Furman University, Greenville, South Carolina-29613, USA.

Dalton Transactions (Cambridge, England : 2003)

|February 4, 2015

Summary

Silver and gold nanoparticles enhance fluorescence in salicylaldehyde solutions. Selective silver enhancement is achieved using ammonia, enabling a sensitive Ag(I) sensor with applications in environmental monitoring.

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Area of Science:

Plasmonics and photonics research.
Metal-enhanced fluorescence (MEF) studies.
Solution-phase chemical sensing.

Background:

Metal-enhanced fluorescence (MEF) typically focuses on solid-state systems.
The role of small organic molecules in solution for MEF is underexplored.
Silver (Ag) and gold (Au) nanoparticles are known to stimulate fluorescence.

Purpose of the Study:

To investigate MEF in aqueous alkaline solutions of salicylaldehyde.
To explore the mechanism of fluorescence enhancement by Ag(I) and Au(III).
To develop a selective sensor for Ag(I) detection.

Main Methods:

In situ synthesis of aggregated silver and gold nanoparticles.
Ageing of aqueous alkaline salicylaldehyde solutions with metal ions.
Spectroscopic analysis of fluorescence enhancement.
Selective detection using imine bond formation with ammonia/amines.

Main Results:

Aqueous alkaline salicylaldehyde exhibits significant fluorescence enhancement with Ag(I) or Au(III) after two days.
Enhanced fluorescence is attributed to increased scattering cross-section and the lightning rod effect of metal aggregates.
Selective fluorescence enhancement by silver alone is achieved via imine formation, eliminating gold-induced enhancement.
A highly selective Ag(I) sensor with a low limit of detection (LOD) was developed.

Conclusions:

Aqueous solutions of salicylaldehyde demonstrate robust MEF with Ag and Au nanoparticles.
The lightning rod effect and scattering properties of metal aggregates are key to MEF.
Imine formation provides a mechanism for selective silver detection, enabling a novel Ag(I) sensor.
This approach offers a cost-effective and sensitive method for environmental monitoring of Ag(I).