Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gas Chromatography: Sample Injection Systems01:08

Gas Chromatography: Sample Injection Systems

2.1K
In gas chromatography, the sample is introduced as a vapor plug into the carrier gas stream for high efficiency and resolution. A microsyringe injects the sample solution into a heated sample port, vaporizing it and mixing it with the carrier gas. This process is important to ensure the sample is properly prepared for analysis. Thermally sensitive samples can be injected directly into the column and volatilized by slowly increasing the column temperature.
Two primary injection methods are used...
2.1K
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

2.1K
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
2.1K
High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

3.7K
High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
3.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Automatic Assay Preparation Platform (A2P2) for Real-Time Critical Quality Attributes Monitoring of Cell Culture Samples.

Biotechnology journal·2025
Same author

Accelerating attribute-focused process and product development through the development and deployment of autonomous process analytical technology platform system.

Biotechnology and bioengineering·2024
See all related articles

Related Experiment Video

Updated: Apr 17, 2026

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor
09:49

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

Published on: April 6, 2016

8.6K

Micro sequential injection system as the interfacing device for process analytical applications.

Chao-Hsiang Richard Wu1, Siowfong Wee

  • 1Analytical Core Services, Amgen Inc., One Amgen Center Drive, Thousand Oaks, CA, 91320.

Biotechnology Progress
|February 4, 2015
PubMed
Summary

Scientists developed an automated sample preparation system for real-time monitoring of amino acids in bioreactors. This platform enables precise control over bioprocesses, ensuring optimal cell culture conditions and product quality.

Keywords:
amino acidsat-line real-time monitoringbioreactor monitoringmammalian cell culturemicro sequential injectiontherapeutic antibodies

More Related Videos

Measuring the Time-Evolution of Nanoscale Materials with Stopped-Flow and Small-Angle Neutron Scattering
07:53

Measuring the Time-Evolution of Nanoscale Materials with Stopped-Flow and Small-Angle Neutron Scattering

Published on: August 6, 2021

2.8K
Automated 90Sr Separation and Preconcentration in a Lab-on-Valve System at Ppq Level
08:53

Automated 90Sr Separation and Preconcentration in a Lab-on-Valve System at Ppq Level

Published on: June 6, 2018

8.5K

Related Experiment Videos

Last Updated: Apr 17, 2026

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor
09:49

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

Published on: April 6, 2016

8.6K
Measuring the Time-Evolution of Nanoscale Materials with Stopped-Flow and Small-Angle Neutron Scattering
07:53

Measuring the Time-Evolution of Nanoscale Materials with Stopped-Flow and Small-Angle Neutron Scattering

Published on: August 6, 2021

2.8K
Automated 90Sr Separation and Preconcentration in a Lab-on-Valve System at Ppq Level
08:53

Automated 90Sr Separation and Preconcentration in a Lab-on-Valve System at Ppq Level

Published on: June 6, 2018

8.5K

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Process Engineering

Background:

  • Maintaining optimal bioreactor conditions is crucial for controlling biological product quality and quantity.
  • Amino acids are essential substrates for cell culture and their levels must be monitored.
  • Excess free amino acids can negatively impact cell growth and product yield.

Purpose of the Study:

  • To develop a compact and robust automated sample preparation platform for bioreactor amino acid monitoring.
  • To enable real-time, at-line analysis of amino acids for bioprocess control.
  • To facilitate rapid decision-making for bioprocess scientists.

Main Methods:

  • Development of a micro sequential injection (μSI) system for automated sample preparation.
  • Integration of the μSI system with an ultra-performance liquid chromatography (UPLC) system.
  • Demonstration of real-time amino acid separation and quantitation in bioreactor samples.

Main Results:

  • Successful implementation of an automated amino acid sample preparation protocol.
  • Real-time amino acid analysis results were achieved, enabling at-line monitoring.
  • The μSI system demonstrated a platform-like capability for future sample preparation methods.

Conclusions:

  • The developed μSI system provides a feasible solution for real-time bioreactor amino acid monitoring.
  • This technology can support the establishment of automatic feedback control mechanisms for bioprocesses.
  • The system is broadly applicable for process analytical technology (PAT) applications in biomanufacturing.