Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Patient-reported outcomes after facial skin cancer surgery: A prospective multicentre cohort study.

Journal of the European Academy of Dermatology and Venereology : JEADV·2026

Same author

A Humanized Bone Metastasis Model: <i>Ex Vivo</i> and <i>In Vivo</i> Applications.

Tissue engineering. Part C, Methods·2026

Same author

Preoperative dual antiplatelet therapy increases risk after urgent coronary bypass surgery: A Netherlands heart registration study.

JTCVS open·2025

Same author

Digital Orthodontic Setups in Orthognathic Surgery: Evaluating Predictability and Precision of the Workflow in Surgical Planning.

Journal of clinical medicine·2025

Same author

Sex-disparities in pediatric and young adult patients with psoriasis treated with biologics: differences in adverse events and disease activity.

The Journal of dermatological treatment·2025

Same author

Periodontal changes after haematopoietic cell transplantation and the role of conditioning regimen intensity.

Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer·2025

Search research articles

Related Experiment Video

Updated: Apr 17, 2026

Expanding Nanopatterned Substrates Using Stitch Technique for Nanotopographical Modulation of Cell Behavior

Expanding Nanopatterned Substrates Using Stitch Technique for Nanotopographical Modulation of Cell Behavior

Published on: December 8, 2016

Bone marrow-derived mesenchymal cells feature selective migration behavior on submicro- and nano-dimensional

Alexey Klymov¹, Ewald M Bronkhorst¹, Joost Te Riet²

¹Department of Biomaterials, Radboud University Medical Center, Nijmegen, The Netherlands.

Acta Biomaterialia

|February 4, 2015

Summary

Cells can distinguish between different nanopatterns, actively avoiding or being attracted to specific surface topographies. This cell behavior on biomaterials is key for regenerative medicine applications.

Keywords:

Cell–surface interactions Migration Nanotopography Proliferation Surface topography

More Related Videos

Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates

Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates

Published on: June 17, 2016

Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix

Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix

Published on: February 9, 2015

Related Experiment Videos

Last Updated: Apr 17, 2026

Expanding Nanopatterned Substrates Using Stitch Technique for Nanotopographical Modulation of Cell Behavior

Expanding Nanopatterned Substrates Using Stitch Technique for Nanotopographical Modulation of Cell Behavior

Published on: December 8, 2016

Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates

Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates

Published on: June 17, 2016

Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix

Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix

Published on: February 9, 2015

Area of Science:

Biomaterials Science
Cell Biology
Regenerative Medicine

Background:

Cellular interaction with biomaterials is crucial for tissue engineering.
Understanding how cells respond to nanoscale surface features is essential for designing effective implants and scaffolds.

Purpose of the Study:

To investigate if cells possess an intrinsic ability to recognize and respond to nanopatterned surfaces.
To determine cellular affinity towards various nanoscale topographies on a multi-patterned biochip.

Main Methods:

Fabrication of a multi-patterned biochip with 36 distinct surfaces (squares and grooves) ranging from 10 to 1000 nm.
Culture of rat bone marrow-derived mesenchymal cells on the biochip to observe cellular accumulation and migration.
Utilized regression analysis to quantify cellular responses to different nanopatterns.

Main Results:

Significant differences in cellular affinity were observed across tested nanopatterns.
Square patterns, especially larger ones, demonstrated cell-repelling properties (up to 40% fewer cells).
Nano-grooved patterns with a 1:3 ridge-to-groove ratio repelled cells, while 3:1 ratios showed partial cell attraction.

Conclusions:

Cells actively avoid or migrate towards surfaces with specific nanometric topographies.
The observed effects are driven by selective cell migration, not proliferation.
Multi-patterned biochips offer enhanced evaluation of cell behavior compared to uniform surfaces, with potential applications in regenerative medicine.