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Updated: Apr 17, 2026

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children
Published on: December 31, 2017
Evaluation of human and microbial DNA content in subgingival plaque samples collected by paper points or curette
P J Pérez-Chaparro1, P M Duarte1, C M Pannuti2
1Department of Periodontology, Dental Research Division, Guarulhos University, Sao Paulo, Brazil.
Abstract:
Host DNA may adversely affect metagenomic studies focusing on the prokaryotic microbiota. This study compared the levels of host DNA in subgingival plaque collected by paper points and curette, using quantitative PCR. Lower proportions of host DNA and higher proportions of bacterial DNA were recovered from samples collected with curettes.
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