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Flame Photometry: Lab

Flame Photometry: Lab

In a flame photometer, when a solution like potassium chloride is aspirated into the flame, the solvent evaporates, leaving behind dehydrated salt. This salt dissociates into free gaseous atoms in their ground state. Some of these atoms absorb energy from the flame, leading to their excitation. The excited atoms return to the ground state, emitting photons at characteristic wavelengths. Because only electronic transitions are involved, the resulting emission lines are very narrow. The intensity...

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Related Experiment Video

Updated: Apr 17, 2026

Measurement of Vacuolar and Cytosolic pH In Vivo in Yeast Cell Suspensions

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Published on: April 19, 2013

Total cellular Ca2+ measurements in yeast using flame photometry.

Renata Tisi¹, Enzo Martegani¹, Rogelio L Brandão²

¹Dipartimento di Biotecnologie e Bioscienze, Università di Milano-Bicocca, Milan, Italy;

Cold Spring Harbor Protocols

|February 4, 2015

Summary

This study details a method for measuring total cellular calcium in yeast using a photoelectric flame photometer. This technique analyzes the light emitted by calcium when introduced into a flame to determine its concentration.

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Area of Science:

Analytical Chemistry
Biochemistry
Cell Biology

Background:

Photoelectric flame photometry is a standard technique for quantifying metal ion concentrations in solution.
Accurate measurement of intracellular metal ions, such as calcium, is crucial for understanding cellular processes.

Purpose of the Study:

To describe a protocol for the accurate determination of total cellular calcium concentrations in yeast.
To adapt photoelectric flame photometry for biological sample analysis.

Main Methods:

Utilizing a photoelectric flame photometer to measure light intensity.
Spraying yeast solutions into a nonluminous flame under controlled conditions.
Quantifying calcium based on maximal light emission at 622 nm (orange flame).

Main Results:

The described protocol allows for the reliable measurement of total cellular calcium in yeast.
The maximal emission wavelength for calcium was confirmed at 622 nm.

Conclusions:

Photoelectric flame photometry is a viable and effective method for quantifying total cellular calcium in yeast.
This protocol provides a foundation for further studies on calcium's role in yeast physiology.