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Related Experiment Video

Updated: Apr 17, 2026

Assay for Adhesion and Agar Invasion in S. cerevisiae
04:36

Assay for Adhesion and Agar Invasion in S. cerevisiae

Published on: November 8, 2006

15.6K

Biofilm/Mat assays for budding yeast.

Paul J Cullen1

  • 1Department of Biological Sciences, State University of New York at Buffalo, Buffalo, New York 14260.

Cold Spring Harbor Protocols
|February 4, 2015
PubMed
Summary

Researchers developed new biofilm/mat assays to study how the Flo11 protein in yeast influences social behavior and virulence. These methods assess cell adhesion and architecture on various surfaces, aiding microbial research.

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Area of Science:

  • Microbiology
  • Mycology
  • Cell Biology

Background:

  • Microbial biofilms and mats are crucial for survival under nutrient limitation.
  • Fungal pathogens utilize biofilm formation for virulence.
  • The flocculin Flo11 protein in budding yeast mediates cell-cell and cell-substrate adhesion in mats.

Purpose of the Study:

  • To develop and validate biofilm/mat assays for studying the role of Flo11.
  • To evaluate the impact of surface rigidity on mat formation and architecture.
  • To quantify cell adherence to plastic surfaces.

Main Methods:

  • Culturing yeast on surfaces with varying rigidity to assess expansion and 3D architecture.
  • Exposing cells to plastic surfaces to measure adherence.
  • Utilizing biofilm/mat assays to analyze Flo11 function.

Main Results:

  • The developed assays effectively evaluate the role of Flo11 in mat formation.
  • Surface rigidity influences yeast mat expansion and architecture.
  • Quantifiable data on cell adherence was obtained.

Conclusions:

  • The novel biofilm/mat assays are broadly applicable for studying microbial social behavior.
  • These assays provide insights into the mechanisms of Flo11-mediated adhesion and mat development.
  • The methods facilitate research on fungal pathogen virulence and biofilm formation.

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