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Updated: Oct 9, 2026

Isolation and Primary Culture of Rat Hepatic Cells
Published on: June 29, 2012
Evidence that the production of acetate in rat hepatocytes is a predominantly cytoplasmic process
B Crabtree1, M J Souter, S E Anderson
1Rowett Research Institute, Bucksburn, Aberdeen, U.K.
Abstract:
By using [1-14C]butyrate, the fluxes of butyrate to acetate and fatty acids were measured in rat hepatocytes. Both fluxes were inhibited to a similar extent by (-)-hydroxycitrate, with no significant effect on butyrate uptake. These results indicate that acetate formation takes place in the cytoplasm, presumably via ATP-stimulated acetyl-CoA hydrolase. Since acetate formation occurred despite a net uptake of acetate, the results are also consistent with the operation of a substrate cycle between acetate and acetyl-CoA, recently proposed by other workers, and suggest that this cycle is cytoplasmic.
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