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Updated: Apr 17, 2026
Influenza A Virus Studies in a Mouse Model of Infection
Published on: September 7, 2017
Basic biological characterization of feline morbillivirus
Rie Koide1, Shoichi Sakaguchi, Takayuki Miyazawa
1Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto 606-8507, Japan.
Abstract:
Feline morbillivirus (FmoPV) is an emerging virus that was recently discovered in domestic cats with chronic nephritis. Despite the potential role of FmoPV in chronic nephritis, little is known about its biological characteristics. In this study, we established a quantitative assay of FmoPV by using an indirect immunofluorescence technique. Viral titers of FmoPV were determined in one week. Treatment with polybrene® or trypsin which was previously used in virus isolation did not augment the virus titers. FmoPV was notably stable at 4°C, retaining high titers for at least 12 days. Heat-treatment at 60°C and 70°C effectively inactivated FmoPV in 10 and 2 min, respectively. The biological characteristics of FmoPV reported here will be beneficial for establishing an efficient virus isolation method and will provide important information to take a measure to reduce the risk of FmoPV infection.
Insights
Feline morbillivirus (FmoPV) is an emerging feline virus. This study details FmoPV
Area of Science:
- Veterinary Virology
- Emerging Infectious Diseases
- Feline Pathology
Background:
- Feline morbillivirus (FmoPV) is an emerging virus implicated in chronic nephritis in domestic cats.
- Limited information exists regarding the biological characteristics of FmoPV, hindering effective management and control strategies.
Purpose of the Study:
- To establish a quantitative assay for FmoPV.
- To characterize the stability and inactivation parameters of FmoPV.
Main Methods:
- Development of a quantitative assay using indirect immunofluorescence.
- Determination of viral titers over time.
- Assessment of FmoPV stability at 4°C and inactivation by heat treatments.
Main Results:
- A quantitative indirect immunofluorescence assay for FmoPV was successfully established.
- FmoPV demonstrated significant stability at 4°C, maintaining high titers for at least 12 days.
- Heat treatment at 60°C inactivated FmoPV within 10 minutes, and at 70°C within 2 minutes.
Conclusions:
- The established quantitative assay provides a reliable method for FmoPV titration.
- Understanding FmoPV's stability and heat inactivation profiles is crucial for developing effective virus isolation protocols.
- This research offers vital data for mitigating FmoPV infection risks in feline populations.
