Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Material Composition and Implantation Site Affect in vivo Device Degradation Rate.

bioRxiv : the preprint server for biology·2024
Same author

Engineering a platform for nerve regeneration with direct application to nerve repair technology.

Biomaterials·2019
Same author

Cell response to plasma electrolytic oxidation surface-modified low-modulus β-type titanium alloys.

Colloids and surfaces. B, Biointerfaces·2019
Same author

Investigation of the intrinsic permeability of ice-templated collagen scaffolds as a function of their structural and mechanical properties.

Acta biomaterialia·2018
Same author

Microstructure and in vivo characterization of multi-channel nerve guidance scaffolds.

Biomedical materials (Bristol, England)·2018
Same author

Collagen: a network for regenerative medicine.

Journal of materials chemistry. B·2016
Same journal

Zinc-polydopamine nanozyme promotes mitochondrial biogenesis and alleviates inflammation and muscle atrophy during the perioperative period of surgery.

Journal of materials science. Materials in medicine·2026
Same journal

Immuno-instructive biomaterials for coronary artery disease and myocardial infarction repair.

Journal of materials science. Materials in medicine·2026
Same journal

Effect of topical nanoemulsion of minoxidil on animal hair growth: an in vivo study.

Journal of materials science. Materials in medicine·2026
Same journal

Selective cytotoxicity of zinc peroxide and tetrapodal zinc oxide micro-nanoparticles against breast cancer cells: synthesis, characterization, and therapeutic potential.

Journal of materials science. Materials in medicine·2026
Same journal

Progress and challenges of tantalum-containing biomaterials in regenerative medicine applications.

Journal of materials science. Materials in medicine·2026
Same journal

Synchronizing degradation with regeneration: a model-driven framework for designing biodegradable biomaterials in bone tissue engineering.

Journal of materials science. Materials in medicine·2026
See all related articles

Related Experiment Video

Updated: Apr 17, 2026

Fabrication of Inverted Colloidal Crystal Polyethylene glycol Scaffold: A Three-dimensional Cell Culture Platform for Liver Tissue Engineering
10:18

Fabrication of Inverted Colloidal Crystal Polyethylene glycol Scaffold: A Three-dimensional Cell Culture Platform for Liver Tissue Engineering

Published on: August 27, 2016

10.5K

Ionic solutes impact collagen scaffold bioactivity.

K M Pawelec1, A Husmann, R J Wardale

  • 1Materials Science and Metallurgy Department, Cambridge Centre for Medical Materials, University of Cambridge, Cambridge, CB3 0FS, UK, pawelec.km@gmail.com.

Journal of Materials Science. Materials in Medicine
|February 5, 2015
PubMed
Summary
This summary is machine-generated.

Adding solutes like sodium chloride or sucrose to collagen slurries significantly alters ice-templated scaffold structure and cell attachment. Ionic solutes increase pore size, while non-ionic solutes reduce it, impacting biological function.

More Related Videos

Three-Dimensional In Vitro Biomimetic Model of Neuroblastoma Using Collagen-Based Scaffolds
07:48

Three-Dimensional In Vitro Biomimetic Model of Neuroblastoma Using Collagen-Based Scaffolds

Published on: July 9, 2021

4.1K
Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment
07:12

Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment

Published on: September 7, 2022

3.0K

Related Experiment Videos

Last Updated: Apr 17, 2026

Fabrication of Inverted Colloidal Crystal Polyethylene glycol Scaffold: A Three-dimensional Cell Culture Platform for Liver Tissue Engineering
10:18

Fabrication of Inverted Colloidal Crystal Polyethylene glycol Scaffold: A Three-dimensional Cell Culture Platform for Liver Tissue Engineering

Published on: August 27, 2016

10.5K
Three-Dimensional In Vitro Biomimetic Model of Neuroblastoma Using Collagen-Based Scaffolds
07:48

Three-Dimensional In Vitro Biomimetic Model of Neuroblastoma Using Collagen-Based Scaffolds

Published on: July 9, 2021

4.1K
Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment
07:12

Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment

Published on: September 7, 2022

3.0K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Materials Science

Background:

  • Collagen scaffolds are crucial for tissue engineering.
  • Controlling scaffold architecture is key to function.
  • Ice-templating is a promising method for scaffold fabrication.

Purpose of the Study:

  • To investigate the effect of solutes on ice-templated collagen scaffold structure.
  • To understand how solute type influences scaffold properties and chondrocyte attachment.

Main Methods:

  • Collagen slurries with 0.5 wt% sodium chloride (ionic) or sucrose (non-ionic) were prepared.
  • Ice-templating was used to fabricate scaffolds.
  • Scaffold structure (pore size, fiber morphology) and chondrocyte attachment were analyzed.

Main Results:

  • Ionic solutes (sodium chloride) decreased collagen entanglement, increasing pore size and reducing chondrocyte attachment.
  • Non-ionic solutes (sucrose) slowed ice growth, reducing pore size and enhancing chondrocyte attachment.
  • Solutes significantly altered scaffold morphology and biological response.

Conclusions:

  • Solute addition provides a tunable method to control collagen scaffold architecture via ice-templating.
  • The choice of solute (ionic vs. non-ionic) dictates scaffold properties and cellular interactions.
  • This approach offers a pathway to engineer biomaterials with tailored biological functions.