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Updated: Apr 17, 2026

Novel Sequence Discovery by Subtractive Genomics
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Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

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Methods for the detection and assembly of novel sequence in high-throughput sequencing data

Manuel Holtgrewe1, Leon Kuchenbecker2, Knut Reinert1

  • 1Department of Computer Science, Freie Universität Berlin and Max Planck Institute for Molecular Genetics, Berlin, Germany.

Bioinformatics (Oxford, England)
|February 5, 2015
PubMed
Summary

We developed ANISE and BASIL, novel methods for assembling large insertions from high-throughput sequencing data. ANISE outperforms existing tools, accurately reconstructing novel sequences and improving structural variant detection.

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