Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Gene Regulation in Microbial Communities: Quorum Sensing

Gene Regulation in Microbial Communities: Quorum Sensing

Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...

Bacterial Signaling

Bacterial Signaling

Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...

Regulation of Bacterial Virulence

Regulation of Bacterial Virulence

Pathogenic bacteria employ a range of regulatory mechanisms to modulate the expression of virulence genes in response to environmental and host-derived signals. These mechanisms ensure that virulence factors are expressed only under favorable conditions, thereby optimizing infection and survival strategies.Mechanisms of Virulence RegulationKey regulatory strategies include:Two-Component Systems: These consist of a membrane-bound sensor kinase and a cytoplasmic response regulator. Environmental...

Cholera

Cholera

Cholera is an acute gastrointestinal disease caused by the Gram-negative bacterium Vibrio cholerae. It is transmitted primarily via the fecal-oral route through the ingestion of contaminated water or food.Vibrio cholerae is a motile, Gram-negative bacterium of the family Vibrionaceae, primarily associated with waterborne outbreaks in areas with inadequate sanitation. Although over 200 serogroups of V. cholerae exist, only O1 and O139 are responsible for epidemic cholera. The O1 serogroup,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Discovery and therapeutic delivery of microRNAs targeting deregulated glioblastoma pathways inhibits tumor growth in mice.

The Journal of clinical investigation·2026

Same author

Development of a novel methyl cellulose hydrogel with physiologically relevant controlled ethanol release for cervical dysplasia ablation.

Bioengineering & translational medicine·2026

Same author

<i>Vibrio parahaemolyticus</i> quorum sensing controls phage VP882 transmission.

mBio·2026

Same author

Molecular basis of quorum-sensing signal transduction by CqsS and its inhibition by CqsA the autoinducer synthase.

bioRxiv : the preprint server for biology·2026

Same author

A transcription factor-sRNA-mediated double-negative feedback loop confers pathogen-specific control of quorum-sensing genes.

mBio·2026

Same author

Discovery of a Human Metabolite that Mimics the Bacterial Quorum-Sensing Autoinducer AI-2.

bioRxiv : the preprint server for biology·2026

Search research articles

Related Experiment Video

Updated: Apr 17, 2026

Coincubation Assay for Quantifying Competitive Interactions between Vibrio fischeri Isolates

Coincubation Assay for Quantifying Competitive Interactions between Vibrio fischeri Isolates

Published on: July 22, 2019

Modulating Vibrio cholerae quorum-sensing-controlled communication using autoinducer-loaded nanoparticles.

Hoang D Lu, Alina C Spiegel, Amanda Hurley

¹∥Department of Chemistry and Biochemistry, Rowan University, Glassboro, New Jersey 08028, United States.

|February 5, 2015

Summary

This summary is machine-generated.

Novel nanoparticles deliver bacterial communication signals, enhancing quorum sensing therapies. This approach offers a promising alternative to traditional antibiotics for combating resistance.

Keywords:

CAI-1 Nanoparticle Vibrio cholerae autoinducer drug delivery quorum sensing

More Related Videos

Author Spotlight: Advancing Therapeutics to Treat Vibriosis in Humans and Aquatic Organisms

Author Spotlight: Advancing Therapeutics to Treat Vibriosis in Humans and Aquatic Organisms

Published on: May 31, 2024

Quantification of Violacein in Chromobacterium violaceum and Its Inhibition by Bioactive Compounds

Quantification of Violacein in Chromobacterium violaceum and Its Inhibition by Bioactive Compounds

Published on: August 8, 2025

Related Experiment Videos

Last Updated: Apr 17, 2026

Coincubation Assay for Quantifying Competitive Interactions between Vibrio fischeri Isolates

Coincubation Assay for Quantifying Competitive Interactions between Vibrio fischeri Isolates

Published on: July 22, 2019

Author Spotlight: Advancing Therapeutics to Treat Vibriosis in Humans and Aquatic Organisms

Author Spotlight: Advancing Therapeutics to Treat Vibriosis in Humans and Aquatic Organisms

Published on: May 31, 2024

Quantification of Violacein in Chromobacterium violaceum and Its Inhibition by Bioactive Compounds

Quantification of Violacein in Chromobacterium violaceum and Its Inhibition by Bioactive Compounds

Published on: August 8, 2025

Area of Science:

Microbiology
Nanotechnology
Drug Delivery

Background:

Bacterial antibiotic resistance necessitates novel therapeutic strategies.
Quorum sensing (QS) modulation presents a promising alternative to conventional antibiotics.
Targeting bacterial communication circuits offers a new avenue for antimicrobial therapies.

Purpose of the Study:

To develop a nanoparticle-based delivery system for the Vibrio cholerae quorum sensing signal CAI-1.
To enhance the efficacy of CAI-1 signaling for quorum sensing modulation.
To assess the potential of nanoparticle-delivered CAI-1 as a next-generation therapeutic.

Main Methods:

Utilized Flash NanoPrecipitation to create water-dispersible CAI-1 nanoparticles.
Administered free CAI-1 and CAI-1 nanoparticles to V. cholerae.
Evaluated the diffusion of nanoparticles across in vivo delivery barriers like intestinal mucus.

Main Results:

Nanoparticles delivered CAI-1 with significantly higher activation of V. cholerae quorum sensing responses (5 orders of magnitude).
CAI-1 nanoparticles demonstrated diffusive properties across intestinal mucus barriers.
The nanoparticle formulation enhanced the potency and delivery of the QS signal.

Conclusions:

Nanoparticle-mediated delivery of QS signals is a viable strategy for enhancing antimicrobial therapies.
This approach holds significant promise for developing next-generation medicines to combat antibiotic resistance.
Combining QS modulation with advanced drug delivery systems opens new therapeutic possibilities.