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Published on: March 10, 2020
Enteropathogens detected in a daycare center, Southeastern Brazil: bacteria, virus, and parasite research
Edna Donizetti Rossi Castro1, Marcela Cristina Braga Yassaka Germini1, Joana D'Arc Pereira Mascarenhas2
1Sao Jose do Rio Preto Faculty of Medicine, Sao Paulo, Brazil.
Insights
This study investigated enteropathogens in daycare children, finding no direct link between infections and diarrhea. Intestinal parasites were associated with eating outside the home.
Area of Science:
- Microbiology
- Infectious Diseases
- Pediatrics
Background:
- Enteropathogens pose a significant health risk to children in congregate settings like daycare centers.
- Understanding the prevalence and types of enteropathogens is crucial for implementing effective control measures.
Purpose of the Study:
- To determine the prevalence and etiological profile of enteropathogens in children attending a daycare center.
- To investigate potential associations between enteropathogen presence and diarrheal symptoms.
Main Methods:
- Stool samples were collected from 100 children at a government daycare center in São José do Rio Preto, Brazil.
- Samples were analyzed for bacterial and viral enteropathogens, as well as intestinal parasites.
- Statistical analysis was performed to identify associations between pathogen presence and clinical symptoms or behavioral factors.
Main Results:
- Bacteria were isolated from 99% of fecal samples, with Escherichia coli being the most common.
- Norovirus and Astrovirus were detected in children with diarrhea, exclusively in urban residents.
- Intestinal parasites including Giardia lamblia and hookworm were observed, with a significant association between parasite presence and eating outside the home/daycare.
Conclusions:
- Intestinal infections by the identified pathogens did not appear to contribute significantly to diarrhea or other symptoms in this daycare population.
- The diversity of enteropathogens may be influenced by Brazil's varied geographical, social, economic, and climatic factors.
- Further research is needed to understand the complex interplay of factors influencing enteropathogen dynamics in childcare settings.
Introduction:
The objective of this study was to determine the prevalence and etiological profile of enteropathogens in children from a daycare center.
Methods:
From October 2010 to February 2011 stool samples from 100 children enrolled in a government daycare center in the municipality of São José do Rio Preto, in the state of São Paulo, were collected and analyzed.
Results:
A total of 246 bacteria were isolated in 99% of the fecal samples; 129 were in the diarrheal group and 117 in the non-diarrheal group. Seventy-three strains of Escherichia coli were isolated, 19 of Enterobacter, one of Alcaligenes and one of Proteus. There were 14 cases of mixed colonization with Enterobacter and E. coli. Norovirus and Astrovirus were detected in children with clinical signs suggestive of diarrhea. These viruses were detected exclusively among children residing in urban areas. All fecal samples were negative for the presence of the rotavirus species A and C. The presence of Giardia lamblia, Entamoeba coli, Endolimax nana and hookworm was observed. A significant association was found between food consumption outside home and daycare center and the presence of intestinal parasites.
Conclusions:
For children of this daycare center, intestinal infection due to pathogens does not seem to have contributed to the occurrence of diarrhea or other intestinal symptoms. The observed differences may be due to the wide diversity of geographical, social and economic characteristics and the climate of Brazil, all of which have been reported as critical factors in the modulation of the frequency of different enteropathogens.
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