Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Gestational lipid profile partially mediates adverse obstetric outcomes associated with polycystic ovary syndrome: a multicentre cohort study in China.

Lipids in health and disease·2026

Same author

Inhibitory effect and mechanism of Nb<sub>2</sub>C nanochaperone on bovine serum albumin unfolding.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026

Same author

Transcranial direct current stimulation for perioperative depression in breast cancer surgery: a randomized controlled trial.

Translational psychiatry·2026

Same author

Genetic inference of the etiological crosstalk between primary glaucomas and retinal vascular occlusions.

Advances in ophthalmology practice and research·2026

Same author

Variations of Alloying Site Density in Pd<sub>1</sub>Cu Single-Atom Alloy Catalysts Lead to Shifted Product Yields in Electrochemical CO Reduction.

Angewandte Chemie (International ed. in English)·2026

Same author

MGA-CLIP: A Multigranularity Attribution Framework for Cross-Modal Explainability in CLIP.

IEEE transactions on neural networks and learning systems·2026

Search research articles

Related Experiment Video

Updated: Apr 17, 2026

Neural Activity Propagation in an Unfolded Hippocampal Preparation with a Penetrating Micro-electrode Array

Neural Activity Propagation in an Unfolded Hippocampal Preparation with a Penetrating Micro-electrode Array

Published on: March 27, 2015

Multi-electrode array capable of supporting precisely patterned hippocampal neuronal networks.

Tianyi Zhou¹, Susan F Perry, Yevgeny Berdichevsky

¹Department of Electrical and Computer Engineering, Lehigh University, Bethlehem, PA, 18015, USA, tiz209@lehigh.edu.

Biomedical Microdevices

|February 6, 2015

Summary

This study uses dielectrophoresis (DEP) and microstructures to precisely position mouse hippocampal neurons on multi-electrode arrays (MEAs). This technique enables effective neuronal network recording and stimulation, demonstrating functional neural activity.

More Related Videos

Author Spotlight: Advancing Genetic Epilepsy Studies with Multi-Electrode Array-Based Long-Term Electrophysiological Monitoring of Human Brain Assembloids

Author Spotlight: Advancing Genetic Epilepsy Studies with Multi-Electrode Array-Based Long-Term Electrophysiological Monitoring of Human Brain Assembloids

Published on: September 27, 2024

Author Spotlight: Advancing Large-Scale Neural Dynamics Through HD-MEA Technology

Author Spotlight: Advancing Large-Scale Neural Dynamics Through HD-MEA Technology

Published on: March 8, 2024

Related Experiment Videos

Last Updated: Apr 17, 2026

Neural Activity Propagation in an Unfolded Hippocampal Preparation with a Penetrating Micro-electrode Array

Neural Activity Propagation in an Unfolded Hippocampal Preparation with a Penetrating Micro-electrode Array

Published on: March 27, 2015

Author Spotlight: Advancing Genetic Epilepsy Studies with Multi-Electrode Array-Based Long-Term Electrophysiological Monitoring of Human Brain Assembloids

Author Spotlight: Advancing Genetic Epilepsy Studies with Multi-Electrode Array-Based Long-Term Electrophysiological Monitoring of Human Brain Assembloids

Published on: September 27, 2024

Author Spotlight: Advancing Large-Scale Neural Dynamics Through HD-MEA Technology

Author Spotlight: Advancing Large-Scale Neural Dynamics Through HD-MEA Technology

Published on: March 8, 2024

Area of Science:

Neuroscience
Bioengineering
Materials Science

Background:

Accurate placement of hippocampal neurons on multi-electrode arrays (MEAs) is crucial for targeted recording and stimulation.
Existing MEA fabrication methods require improvement in neuronal cell adhesion and network formation.

Purpose of the Study:

To develop a method for precise positioning of primary mouse hippocampal neurons on MEAs using dielectrophoresis (DEP).
To create patterned neuronal networks on MEAs using microstructures.
To optimize MEA surface pretreatment for enhanced neuronal cell compatibility.

Main Methods:

Utilized positive dielectrophoresis (DEP) to actively recruit hippocampal neurons to MEA electrodes.
Fabricated microstructures (chambers and trenches) to define patterned neuronal networks.
Developed and applied effective pretreatment methods for SU-8 epoxy MEA fabrication to improve cytocompatibility.

Main Results:

Successfully positioned primary mouse hippocampal neurons on MEA electrodes using DEP.
Demonstrated the formation of patterned neuronal networks with defined microstructures.
Achieved functional recording of spontaneous and stimulated neuronal potentials from primary hippocampal neurons.
Observed the propagation of evoked neuronal bursts between electrodes, confirming network functionality.

Conclusions:

The developed DEP-based method enables precise neuronal positioning on MEAs for targeted electrophysiological studies.
Optimized MEA surface pretreatment enhances neuronal adhesion and network formation.
The novel MEA system successfully records and stimulates neuronal activity, paving the way for advanced neural interface applications.