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Development of a peptide nucleic acid-based multiprobe real-time PCR method targeting the hsp65 gene for
Kijeong Kim1, Byoung-Jun Kim2, Tae Sun Shim3
1Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
Journal of Clinical Microbiology
|February 6, 2015
Abstract:
Recently, the need to distinguish between members of the Mycobacterium abscessus group has gained increasing attention. Here, we introduced a novel peptide nucleic acid (PNA) real-time PCR method targeting the hsp65 gene in order to distinguish between four subspecies within the M. abscessus group (M. abscessus and 3 types of M. massiliense).

