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Related Experiment Video

Updated: Apr 17, 2026

High Content Screening in Neurodegenerative Diseases

High Content Screening in Neurodegenerative Diseases

Published on: January 6, 2012

Advances in laser scanning imaging cytometry for high-content screening.

Paul G Wylie¹, David J Onley, Anne F Hammerstein

¹TTP Labtech Limited , Melbourn, Hertfordshire, United Kingdom .

Assay and Drug Development Technologies

|February 6, 2015

Summary

Laser scanning imaging cytometry (LSIC) enhances drug discovery by enabling rapid, high-content screening of compound effects on cellular functions. This technology supports phenotypic screening, cell health assays, and advanced 3D cell models.

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Area of Science:

Biotechnology
Drug Discovery
Cellular Imaging

Background:

High-content screening (HCS) revolutionized drug discovery workflows over a decade ago.
Cell-based assays are crucial for target identification, screening, lead optimization, and toxicity assessment.
Laser scanning imaging cytometry (LSIC) is a key HCS technology enabling detailed cellular analysis.

Purpose of the Study:

To review advancements in LSIC technology.
To showcase the application of LSIC in various drug discovery assays.
To highlight real-world case studies demonstrating LSIC's utility.

Main Methods:

LSIC utilizes laser scanning excitation with an F-theta scan lens for rapid, high-resolution whole-well imaging.
Modern LSIC systems incorporate multiple lasers and detectors for spectral analysis, enabling multiplexing.
The technology is applied to a wide range of fluorescently labeled cell-based assays.

Main Results:

LSIC allows for the identification and quantification of compound effects on multiple cellular functions.
Increased multiplexing capabilities enhance the depth of cellular analysis.
LSIC is extensively used for phenotypic screening, including cell health and RNA interference (RNAi) studies.

Conclusions:

LSIC is a powerful tool for characterizing chemical libraries and accelerating drug discovery.
Its application in phenotypic screening, particularly with 3D cell models, is expanding.
Advancements in LSIC technology continue to improve throughput and analytical capabilities for drug development.