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Improved data-dependent acquisition for untargeted metabolomics using gas-phase fractionation with staggered mass

Zhixiang Yan1, Ru Yan

  • 1State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau , Taipa, Macao 999078, China.

Analytical Chemistry
|February 6, 2015
PubMed
Summary

This study introduces staggered gas-phase fractionation (sGPF), an improved data-dependent acquisition method for untargeted metabolomics. sGPF enhances MS2 coverage by enabling more homogeneous precursor ion selection, significantly boosting metabolite identification in complex samples.

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