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Classification of contraction bands using immunohistochemistry

Satomu Morita1, Satoshi Furukawa, Katsuji Nishi

  • 1From the Department of Legal Medicine, Shiga University of Medical Science, Seta Tsukinowa-cho, Otsu City, Shiga, Japan.

The American Journal of Forensic Medicine and Pathology
|February 6, 2015
PubMed

Insights

Pathological contraction bands (CBs) in the heart are diverse and poorly understood. This study proposes a new classification for CB necrosis using complement component C9 (CCC9) and Sirtuin1, aiding research into their causes.

Area of Science:

  • Cardiovascular Pathology
  • Forensic Medicine
  • Immunohistochemistry

Background:

  • Pathological contraction bands (CBs) represent a necrosis pattern in the myocardium.
  • The diverse forms and varied causes of CBs have hindered detailed study.
  • Current classification distinguishes artifactual CB from CB necrosis.

Purpose of the Study:

  • To identify and classify different forms of myocardial contraction bands.
  • To investigate the expression and distribution of complement component C9 (CCC9) and Sirtuin1 in CBs.
  • To propose a novel immunohistochemistry-based classification scheme for CB necrosis.

Main Methods:

  • Analysis of cardiac tissues from 30 forensic autopsy cases with significant CBs.
  • Immunohistochemical examination of complement component C9 (CCC9) and Sirtuin1 expression and distribution.
  • Correlation of CB classification with agonal situations (e.g., high temperature, myocardial infarction, CPR, hypothermia).

Main Results:

  • A new classification scheme for CBs based on CCC9 and Sirtuin1 expression was developed.
  • This classification correlated significantly with various agonal conditions.
  • CB necrosis was reliably detected using CCC9 immunostaining.

Conclusions:

  • Advocacy for an immunohistochemistry-based classification scheme for pathological contraction bands.
  • The proposed scheme aids in differentiating CB types and understanding causative mechanisms.
  • CCC9 immunostaining is a valuable tool for detecting CB necrosis.

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