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Patch Clamp

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

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Related Experiment Video

Updated: Apr 17, 2026

A Computer-assisted Multi-electrode Patch-clamp System

A Computer-assisted Multi-electrode Patch-clamp System

Published on: October 18, 2013

An optogenetics- and imaging-assisted simultaneous multiple patch-clamp recording system for decoding complex neural

Guangfu Wang¹, Daniel R Wyskiel¹, Weiguo Yang²

¹Department of Pharmacology, University of Virginia, Charlottesville, Virginia, USA.

Nature Protocols

|February 6, 2015

Summary

This summary is machine-generated.

We developed a novel octuple patch-clamp recording method for detailed analysis of neuronal circuits. This technique enables simultaneous physiological and anatomical characterization of multiple neurons, advancing brain function research.

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Electrophysiological and Morphological Characterization of Neuronal Microcircuits in Acute Brain Slices Using Paired Patch-Clamp Recordings

Electrophysiological and Morphological Characterization of Neuronal Microcircuits in Acute Brain Slices Using Paired Patch-Clamp Recordings

Published on: January 10, 2015

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Last Updated: Apr 17, 2026

A Computer-assisted Multi-electrode Patch-clamp System

A Computer-assisted Multi-electrode Patch-clamp System

Published on: October 18, 2013

One-channel Cell-attached Patch-clamp Recording

One-channel Cell-attached Patch-clamp Recording

Published on: June 9, 2014

Electrophysiological and Morphological Characterization of Neuronal Microcircuits in Acute Brain Slices Using Paired Patch-Clamp Recordings

Electrophysiological and Morphological Characterization of Neuronal Microcircuits in Acute Brain Slices Using Paired Patch-Clamp Recordings

Published on: January 10, 2015

Area of Science:

Neuroscience
Cellular Biology
Systems Biology

Background:

Understanding neuronal circuitry is crucial for brain function and dysfunction research.
Dissecting neuronal circuits requires analyzing synaptic connections and neuron morphology.
Current methods face challenges in simultaneously recording and identifying multiple neurons.

Purpose of the Study:

To present a method for stable simultaneous octuple patch-clamp recordings.
To enable functional and morphological analysis of neuronal circuits.
To optimize a combined microscopy and optogenetics-assisted recording system.

Main Methods:

Stable simultaneous octuple patch-clamp recordings in rodent tissue slices.
Combined single- and two-photon microscopy with optogenetics and imaging.
Refinements in mechanics, electronics, optics, and software for enhanced recording.

Main Results:

Physiological analysis of synaptic interconnections among 4-8 simultaneously recorded neurons.
Anatomical identification of >85% of interneurons and >99% of principal neurons.
System setup and operation achievable within 3-4 days.

Conclusions:

The developed octuple patch-clamp method facilitates neuronal circuit dissection.
The system allows comprehensive physiological and anatomical characterization of neurons.
This technique is adaptable to various model organisms and advances neuroscience research.