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Published on: December 4, 2015
Altered splenic miRNA expression profile in H1N1 swine influenza
Liangzong Huang1, Jun Ma, Yankuo Sun
1College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China.
Abstract:
Previous studies have demonstrated the key regulatory roles played by microRNAs (miRNAs) in influenza virus-host interactions. To gain more insight into the contribution of miRNAs to the host immune response, spleen tissues from mice infected with A/Swine/GD/2/12 (H1N1) virus were harvested 5 days postinfection, and miRNA deep sequencing was performed. The results showed that 50 miRNAs were modulated. Interestingly, pathway analysis of miRNAs and targets showed that upregulated miR-124-3p interacts with innate immune-related pathways such as the Toll-like receptor pathway, RIG-I-like receptor signaling pathway, NOD-like receptor signaling pathway and JAK-STAT signaling pathway, and this might play a major role in the anti-inflammatory response. Further understanding of the roles played by these miRNAs in influenza virus infection will provide new insights into host-pathogen interactions.
Insights
MicroRNAs (miRNAs) are key in influenza virus-host interactions. Upregulated miR-124-3p in mice infected with H1N1 influenza may play a significant role in the anti-inflammatory immune response.
Area of Science:
- Immunology
- Virology
- Genetics
Background:
- MicroRNAs (miRNAs) are crucial regulators of gene expression.
- miRNAs play significant roles in host-pathogen interactions, including influenza virus infections.
- Understanding miRNA involvement in the immune response to influenza is vital.
Purpose of the Study:
- To investigate the role of miRNAs in the host immune response to H1N1 influenza virus infection.
- To identify specific miRNAs modulated during influenza virus infection and their potential functions.
Main Methods:
- Spleen tissues were collected from mice 5 days post-infection with A/Swine/GD/2/12 (H1N1) virus.
- miRNA deep sequencing was employed to profile miRNA expression.
- Bioinformatic pathway analysis was conducted on modulated miRNAs and their predicted targets.
Main Results:
- Deep sequencing identified 50 modulated miRNAs in response to H1N1 influenza infection.
- Upregulated miR-124-3p was found to interact with key innate immune pathways.
- These pathways include Toll-like receptor, RIG-I-like receptor, NOD-like receptor, and JAK-STAT signaling.
Conclusions:
- miR-124-3p may be a significant factor in the anti-inflammatory immune response during H1N1 influenza infection.
- Further research into miRNA functions during influenza infection can elucidate host-pathogen interactions.
- These findings contribute to understanding the complex interplay between viruses and their hosts.
