Related Experiment Video
Updated: Apr 17, 2026
12:12
Transformation of Probiotic Yeast and Their Recovery from Gastrointestinal Immune Tissues Following Oral Gavage in Mice
Published on: February 8, 2016
13.2K
A simple and efficient genetic transformation method of Ganoderma weberianum
Yu-Ping Zhou1, Min-Hua Chen, Jun-Jie Lu
1Guangzhou Key Laboratory for Functional Study on Stress-Resistant Genes in Plants, School of Life Sciences, Guangzhou University, Guangzhou, 510006, China.
Folia Microbiologica
|February 7, 2015
Summary
A new Agrobacterium tumefaciens-mediated transformation method for Ganoderma weberianum has been established. This efficient technique allows for the expression of genes like hygromycin phosphotransferase in transgenic fungi, aiding molecular genetics research.
Area of Science:
- Mycology
- Molecular Biology
- Biotechnology
Background:
- Ganoderma weberianum is a fungus with potential biotechnological applications.
- Efficient genetic manipulation tools are crucial for studying and improving fungal species.
- Previous methods for genetic transformation of G. weberianum were limited.
Purpose of the Study:
- To establish a reliable Agrobacterium tumefaciens-mediated transformation system for Ganoderma weberianum.
- To enable the expression of foreign genes in G. weberianum for functional analysis.
- To develop a tool for genetic manipulation and molecular studies of G. weberianum.
Main Methods:
- Agrobacterium tumefaciens-mediated transformation using strain GV3101 and expression vector pBI-H1.
- Co-cultivation of fungal mycelia with Agrobacterium lawn on yeast extract agar (YEA) medium at 28°C.
- Selection of transformants using hygromycin B and isolation of single spores for stable lines.
Main Results:
- Successfully established an efficient Agrobacterium-mediated transformation system for G. weberianum.
- Demonstrated efficient expression of hygromycin phosphotransferase (hpt), β-glucuronidase (uidA), and enhanced green fluorescent protein (egfp) genes.
- Achieved high genetic stability in transformants, with over 80% stability after ten subcultures.
Conclusions:
- The developed transformation method is simple, efficient, and provides stable genetic transformants.
- This system serves as a valuable tool for molecular genetics analyses and gene manipulation in G. weberianum.
- Facilitates future research into the functional genomics and biotechnology of G. weberianum.
Related Concept Videos
01:26Transformation
1.4K
Microbial communities are dynamic environments where cell lysis releases free DNA into the surroundings. Other cells can take up this extracellular DNA through a process known as transformation.When a cell incorporates this foreign DNA into its genome, resulting in genetic modification, the process is known as transformation. Cells capable of this process are termed competent. Competence can be natural, as observed in certain bacteria and archaea, or artificially induced in the...
1.4K
01:33Bacterial Transformation
64.0K
In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
64.0K
02:50Transgenic Plants
9.3K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
9.3K