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Flow Cytometry01:23

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM
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Micro flow cytometer with self-aligned 3D hydrodynamic focusing.

Genni Testa1, Gianluca Persichetti1, Romeo Bernini1

  • 1Institute for Electromagnetic Sensing of the Environment (IREA), National Research Council, (CNR), Via Diocleziano 328, 80124 Napoli, Italy.

Biomedical Optics Express
|February 7, 2015
PubMed
Summary
This summary is machine-generated.

A novel micro flow cytometer utilizes 3D hydrodynamic flow focusing to create a self-aligned single-particle stream for efficient microparticle analysis. This device enables precise measurement of microparticles, enhancing flow cytometry applications.

Keywords:
(120.5820) Scattering measurements(230.3990) Micro-optical devices(300.2530) Fluorescence, laser-induced

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Area of Science:

  • Biomedical Engineering
  • Microfluidics
  • Analytical Chemistry

Background:

  • Traditional flow cytometry often requires complex fluidic systems for precise particle alignment.
  • Developing microfluidic devices for single-file particle stream generation is crucial for miniaturized analytical systems.

Purpose of the Study:

  • To develop and validate a micro flow cytometer featuring a single-step 3D hydrodynamic flow focusing mechanism.
  • To demonstrate self-alignment of a particle stream with an integrated optical fiber detection system.
  • To achieve tuneable stream sizes for versatile microparticle analysis.

Main Methods:

  • Fabrication of the microfluidic device using direct micro milling of Polymethyl methacrylate (PMMA) sheets.
  • Implementation of a single-step 3D hydrodynamic flow focusing technique.
  • Integration of an optical fiber-based detection system.
  • Experimental validation using fluorescent microparticles (10-23 μm).

Main Results:

  • Successfully generated a self-aligned, single-file particle stream irrespective of flow rate ratios.
  • Achieved tuneable stream sizes ranging from approximately 18 to 50 μm.
  • Demonstrated accurate alignment and measurement of microparticles with a good coefficient of variance.

Conclusions:

  • The developed micro flow cytometer with 3D hydrodynamic focusing offers a robust and adaptable platform for microparticle analysis.
  • The self-aligning capability simplifies integration with detection systems, paving the way for more compact flow cytometers.
  • The device shows promise for various applications requiring precise microparticle characterization.