Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Reliable five-minute test strip method for identification of Streptococcus pyogenes.

S F Dealler1, L Campbell, K G Kerr

  • 1Department of Microbiology, University of Leeds, UK.

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
|April 1, 1989
PubMed
Summary

A new Strep Strip test accurately identifies Streptococcus pyogenes using pyroglutamyl aminopeptidase and beta-glucosidase tests. This rapid, economical method enhances clinical diagnosis of beta-haemolytic streptococci.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reproductive Modes of Amphibians and Reptiles: Impacts on Conservation and the Development of Assisted Reproductive Technologies.

Advances in experimental medicine and biology·2026
Same author

Co-Producing a Package of Novel Online Resources to Support the Mental Health Needs of People With Persistent Pain: BeeFree.

Musculoskeletal care·2024
Same author

The SelfSTarT intervention for low back pain patients presenting to first contact physiotherapists: A mixed methods service evaluation.

Musculoskeletal care·2024
Same author

Memory support training and lifestyle modifications to promote healthy aging in persons at risk for Alzheimer's disease: a digital application supported intervention (Brain Boosters).

BMC geriatrics·2023
Same author

Understanding pharmaceutical exposure and the potential for effects in marine biota: A survey of bonefish (Albula vulpes) across the Caribbean Basin.

Chemosphere·2023
Same author

The implementation and utility of clinical exome sequencing in a South African infant cohort.

Frontiers in genetics·2023

Area of Science:

  • Microbiology
  • Clinical Diagnostics
  • Bacteriology

Background:

  • Accurate identification of Streptococcus pyogenes is crucial for timely treatment of infections.
  • Existing methods for Streptococcus pyogenes identification can be time-consuming or require specialized equipment.
  • A need exists for a rapid, reliable, and cost-effective diagnostic tool for clinical laboratories.

Purpose of the Study:

  • To evaluate the performance of a novel paper strip test for the rapid identification of Streptococcus pyogenes.
  • To assess the specificity and accuracy of the Strep Strip method in clinical and culture collection isolates.
  • To determine the utility of the combined pyroglutamyl aminopeptidase (PYR) and beta-glucosidase tests for differentiating streptococcal species.

Main Methods:

Related Experiment Videos

  • The Strep Strip method, combining PYR and beta-glucosidase tests on a paper strip, was evaluated.
  • The test was applied to 274 clinical isolates and 237 culture collection isolates of beta-haemolytic streptococci.
  • Results were compared against established identification methods for Streptococcus pyogenes.

    • Main Results:

    • The Strep Strip method demonstrated 100% specificity in identifying Streptococcus pyogenes.
    • The test accurately differentiated Streptococcus pyogenes from other beta-haemolytic streptococci, including six Lancefield group A isolates that were not Streptococcus pyogenes.
    • The beta-glucosidase component of the test successfully distinguished between PYR-positive enterococci and Streptococcus pyogenes in all instances.

    Conclusions:

    • The Strep Strip method provides an economical and accurate approach for the identification of Streptococcus pyogenes in clinical settings.
    • This novel test offers a rapid and reliable alternative for routine laboratory diagnosis.
    • The combined enzymatic tests on the paper strip enhance diagnostic precision for beta-haemolytic streptococci.