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Updated: Apr 17, 2026
Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
Published on: March 16, 2017
A FRET-based ratiometric fluorescent probe for nitroxyl detection in living cells
Huatang Zhang1, Ruochuan Liu, Yi Tan
1Department of Biology and Chemistry, City University of Hong Kong , 83 Tat Chee Avenue, Kowloon, Hong Kong, People's Republic of China.
Abstract:
HNO has recently been found to possess distinct biological functions from NO. Studying the biological functions of HNO calls for the development of sensitive and selective fluorescent probes. Herein, we designed and synthesized a FRET-based ratiometric probe to detect HNO in living cells. Our studies revealed that the probe is capable of detecting HNO in a rapid and ratiometric manner under physiological conditions. In bioimaging studies, the probe displayed a clear color change from blue to green when treated with HNO.
