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Updated: Apr 17, 2026
Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical Methylation and Physical Mass Spectrometry, Nuclear Magnetic Resonance Techniques
Published on: March 24, 2016
Effect of pretreatment on arabinoxylan distribution in wheat bran
Johannes Hell1, Lloyd Donaldson2, Herbert Michlmayr3
1Department of Chemistry, University of Natural Resources and Life Sciences, Konrad-Lorenz-Straße 24, 3430 Tulln, Austria; Department of Food Science and Technology, University of Natural Resources and Life Sciences, Muthgasse 18, Vienna, Austria; Christian Doppler Laboratory for Innovative Bran Biorefinery, Vienna, Austria.
Abstract:
Arabinoxylan is one of the potential key products of a wheat bran based biorefinery. To develop a suitable process for the isolation of arabinoxylans, the effect of different processing approaches needs to be determined. In this work, chemical analysis was supplemented by immunolocalization of arabinoxylan by confocal microscopy, which proved valuable in the assessment of cell-structural changes occurring upon different chemical and mechanical bran treatments. The influences of acid, lye and hydrogen peroxide treatment, ball-milling, extrusion, fermentation and treatment with esterase, xylanase and a combination thereof were investigated. Extensive ball-milling showed the best selectivity for harvesting arabinoxylan. Chemical treatments gave the highest yields, but did so at the cost of selectivity. Fermentative and enzymatic treatments were hampered by coextraction of other compounds.
