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Lysozyme depolymerization of photo-activated chitosan adhesive films.
Damia Mawad1, Charles Warren2, Mathew Barton3
1Department of Materials, Department of Bioengineering and Institute for Biomedical Engineering, Imperial College London, Exhibition Road, London SW7 2AZ, UK.
Carbohydrate Polymers
|February 10, 2015
Summary
Incorporating lysozyme into rose bengal-chitosan films enhances bioadhesive depolymerization after implantation. Laser activation did not impede lysozyme
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Polymer Chemistry
Background:
- Rose bengal-chitosan films demonstrate effective tissue bioadhesion via photoactivation.
- Lysozyme is an enzyme known to depolymerize chitosan.
Purpose of the Study:
- To investigate the effect of lysozyme incorporation on the depolymerization rate of rose bengal-chitosan films.
- To assess the impact of green laser photoactivation on lysozyme activity within the bioadhesive films.
Main Methods:
- Preparation of rose bengal-chitosan films with and without lysozyme.
- In vivo implantation of films in rats for 4 weeks.
- Analysis of film mass loss and degradation products using capillary electrophoresis-mass spectroscopy.
Main Results:
- Lysozyme-loaded films showed a significantly higher mass loss (21%) compared to control films (7%) after 4 weeks.
- Chitosan was observed to degrade into glucosamine and N-acetyl-glucosamine monomers and oligomers.
- Green laser irradiation did not negatively affect the lysozyme-mediated depolymerization process.
Conclusions:
- Lysozyme inclusion is a viable strategy to accelerate the depolymerization of rose bengal-chitosan bioadhesives.
- Photoactivated bioadhesives incorporating lysozyme offer tunable degradation properties for biomedical applications.
