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Replication of IMR-32-adapted JC virus clones in human embryonic kidney cells
Souichi Nukuzuma1, Shigeki Sugiura, Kazuo Nakamichi
1Department of Infectious Diseases, Kobe Institute of Health, 4-6 Minatojima-Nakamachi, Chuo-ku, Kobe, 650-0046.
Abstract:
It has been difficult to study JCV replication because of its restricted host range. In this study, JCV replication was examined using different clones in 293 cells. RT-PCR assay revealed that large T antigen expression in cells transfected with IMR-32-adapted JCVs was significantly greater than in those transfected with Mad-1 or CY. DNA replication assay and viral load verified that the IMR-32-adapted JCVs were replication-competent in 293 cells, but not Mad-1 or CY JCVs. These results suggest that a 293 culture system with IMR-32-adapted JCVs may be a useful tool for assessing replication of JCV in vitro.
