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Updated: Apr 17, 2026

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

Published on: February 27, 2015

Bacterial adhesion force quantification by fluidic force microscopy.

Eva Potthoff¹, Dario Ossola, Tomaso Zambelli

¹Institute of Microbiology, ETH Zurich, Vladimir-Prelog-Weg 4, 8093 Zurich, Switzerland. vorholt@micro.biol.ethz.ch.

|February 10, 2015

Summary

This summary is machine-generated.

We developed a new method using fluidic force microscopy to measure single bacterial cell detachment forces. This technique quantifies bacterial adhesion, crucial for understanding biofilms and infections.

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Last Updated: Apr 17, 2026

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

Published on: February 27, 2015

Introducing Shear Stress in the Study of Bacterial Adhesion

Introducing Shear Stress in the Study of Bacterial Adhesion

Published on: September 2, 2011

Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell

Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell

Published on: August 4, 2015

Area of Science:

Microbiology
Biophysics
Materials Science

Background:

Bacterial adhesion to substrates is critical for survival, biofilm formation, and infection.
Quantifying bacterial detachment forces aids in understanding adhesion mechanisms and their impact on cell physiology.

Purpose of the Study:

To present a novel method for serial, single bacterial cell force spectroscopy.
To quantify adhesion forces between bacteria and substrates using atomic force microscopy combined with microfluidics.

Main Methods:

Utilized fluidic force microscopy (FluidFM) for controlled, reversible bacterial cell immobilization under physiological conditions.
Employed underpressure on a microchanneled cantilever tip for cell attachment.
Performed single-cell force spectroscopy to measure detachment forces.

Main Results:

Achieved robust immobilization forces exceeding state-of-the-art methods for Escherichia coli (4-8 nN).
Demonstrated measurement of contact time and setpoint dependence of adhesion forces for E. coli and Streptococcus pyogenes.
Observed distinct force patterns during sequential detachment of bacteria from a chain.

Conclusions:

FluidFM technology enables quantitative measurements of bacterial adhesion forces.
The method is suitable for studying cell-substrate and cell-cell interactions relevant to biofilms and infection biology.