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Related Experiment Video

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Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

Published on: January 29, 2014

A quantitative lateral flow assay to detect complement activation in blood.

Elizabeth C Schramm¹, Nick R Staten², Zhouning Zhang²

¹Division of Rheumatology, Washington University School of Medicine, St. Louis, MO 63110, USA; Kypha, St. Louis, MO 63108, USA.

Analytical Biochemistry

|February 10, 2015

Summary

A new lateral flow immunoassay accurately measures iC3b in blood, providing a rapid and sensitive tool for assessing complement activation in various inflammatory diseases.

Keywords:

Complement activation Intracerebral hemorrhage (ICH)Lateral flow assay Lupus iC3b biomarker

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Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

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Published on: November 13, 2021

Area of Science:

Immunology
Biochemistry

Background:

The complement system is crucial for innate immunity, with C3 central to its amplification loop.
Excessive complement activation can cause host tissue damage.
Current methods for monitoring complement activation, like total C3 levels, are indirect and insensitive.

Purpose of the Study:

To develop a novel assay for direct measurement of complement activation.
To overcome limitations of existing methods, such as in vitro activation artifacts.
To provide a rapid, point-of-care tool for assessing complement activation.

Main Methods:

Development of a lateral flow immunoassay (LFIA) to measure iC3b.
Assay validation in whole blood, plasma, and serum.
Minimizing sample handling to prevent in vitro complement activation.

Main Results:

The LFIA specifically measures iC3b with minimal cross-reactivity.
Results are obtained within 15 minutes.
Circulating iC3b levels effectively distinguish healthy individuals from those with complement activation-associated diseases.

Conclusions:

The developed LFIA offers an improved method for assessing complement activation.
This assay provides a sensitive and rapid point-of-care diagnostic tool.
It has the potential to facilitate disease monitoring in inflammatory conditions.